tetano
Editor, Senior Moderator
Influenza Other Respir Viruses
. 2021 Feb 26.
doi: 10.1111/irv.12830. Online ahead of print.
Comparison of performance between Fast Track Diagnostics Respiratory Kit and the CDC global reference laboratory for influenza rRT-PCR panel for detection of influenza A and influenza B
Assana Ciss?[SUP] 1 [/SUP], Jennifer Milucky[SUP] 2 [/SUP], Abdoul Kader Ilboudo[SUP] 1 [/SUP], Jessica L Waller[SUP] 2 [/SUP], Brice Bicaba[SUP] 3 [/SUP], Isa?e Medah[SUP] 3 [/SUP], Sara Mirza[SUP] 2 [/SUP], Cynthia G Whitney[SUP] 2 [/SUP], Zekiba Tarnagda[SUP] 1 [/SUP]
Affiliations
Abstract
Background: Reliable diagnostics are a key to identifying influenza infections.
Objectives: Our objectives were to describe the detection of influenza among severe acute respiratory infection (SARI) cases, to compare test results from the Fast Track Diagnostics (FTD) Kit for influenza detection to the Centers for Disease Control (CDC) human influenza virus detection and characterization panel, and to assess seasonality of influenza in Burkina Faso.
Methods: Nasopharyngeal and oropharyngeal specimens from SARI cases (hospitalized patients with fever, cough, and onset in the previous 10 days) were tested using the FTD-33 Kit and the CDC rRT-PCR influenza assays. We assessed sensitivity and specificity of the FTD-33 Kit for detecting influenza A, influenza B, and the influenza A(H1N1)pdm09 strain using the CDC human influenza rRT-PCR panel as the gold standard.
Results: From December 2016 to February 2019, 1706 SARI cases were identified, 1511 specimens were tested, and 211 were positive for influenza A (14.0%) and 100 for influenza B (6.6%) by either assay. Higher influenza circulation occurred between November and April with varying peaks of influenza A and influenza B. Sensitivity of the FTD-33 assay was 91.9% for influenza A, 95.7% for influenza B, and 93.8% for A(H1N1)pdm09 subtype. Specificity was over 99% for all three tests.
Conclusions: Our study indicates that Burkina Faso has one peak of influenza each year which is similar to the Northern Hemisphere and differs from other countries in West Africa. We found high concordance of influenza results between the two assays indicating FTD-33 can be used to reliably detect influenza among SARI cases.
Keywords: Burkina Faso; West Africa; diagnostics; influenza; severe acute respiratory infections.
. 2021 Feb 26.
doi: 10.1111/irv.12830. Online ahead of print.
Comparison of performance between Fast Track Diagnostics Respiratory Kit and the CDC global reference laboratory for influenza rRT-PCR panel for detection of influenza A and influenza B
Assana Ciss?[SUP] 1 [/SUP], Jennifer Milucky[SUP] 2 [/SUP], Abdoul Kader Ilboudo[SUP] 1 [/SUP], Jessica L Waller[SUP] 2 [/SUP], Brice Bicaba[SUP] 3 [/SUP], Isa?e Medah[SUP] 3 [/SUP], Sara Mirza[SUP] 2 [/SUP], Cynthia G Whitney[SUP] 2 [/SUP], Zekiba Tarnagda[SUP] 1 [/SUP]
Affiliations
- PMID: 33638312
- DOI: 10.1111/irv.12830
Abstract
Background: Reliable diagnostics are a key to identifying influenza infections.
Objectives: Our objectives were to describe the detection of influenza among severe acute respiratory infection (SARI) cases, to compare test results from the Fast Track Diagnostics (FTD) Kit for influenza detection to the Centers for Disease Control (CDC) human influenza virus detection and characterization panel, and to assess seasonality of influenza in Burkina Faso.
Methods: Nasopharyngeal and oropharyngeal specimens from SARI cases (hospitalized patients with fever, cough, and onset in the previous 10 days) were tested using the FTD-33 Kit and the CDC rRT-PCR influenza assays. We assessed sensitivity and specificity of the FTD-33 Kit for detecting influenza A, influenza B, and the influenza A(H1N1)pdm09 strain using the CDC human influenza rRT-PCR panel as the gold standard.
Results: From December 2016 to February 2019, 1706 SARI cases were identified, 1511 specimens were tested, and 211 were positive for influenza A (14.0%) and 100 for influenza B (6.6%) by either assay. Higher influenza circulation occurred between November and April with varying peaks of influenza A and influenza B. Sensitivity of the FTD-33 assay was 91.9% for influenza A, 95.7% for influenza B, and 93.8% for A(H1N1)pdm09 subtype. Specificity was over 99% for all three tests.
Conclusions: Our study indicates that Burkina Faso has one peak of influenza each year which is similar to the Northern Hemisphere and differs from other countries in West Africa. We found high concordance of influenza results between the two assays indicating FTD-33 can be used to reliably detect influenza among SARI cases.
Keywords: Burkina Faso; West Africa; diagnostics; influenza; severe acute respiratory infections.