tetano
Editor, Senior Moderator
Genome Announc. 2014 Dec 24;2(6). pii: e01349-14. doi: 10.1128/genomeA.01349-14.
[h=1]Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells.[/h] Mishel P[SUP]1[/SUP], Bychkov D[SUP]1[/SUP], Kallio-Kokko H[SUP]2[/SUP], Valkonen M[SUP]3[/SUP], Kantele A[SUP]3[/SUP], Mattila P[SUP]1[/SUP], Almusa H[SUP]1[/SUP], Jalovaara P[SUP]1[/SUP], Kainov D[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Here, we sequenced the genome of the influenza A/Finland/741 M/2014(H1N1) virus and found that the virus accumulated oseltamivir resistance mutation H275Y in its neuraminidase during propagation in cell culture. This indicates that propagation in cell culture modifies virus genomes. The instability of influenza genomes should be taken into consideration during drug-sensitivity studies.
Copyright ? 2014 Mishel et al.
PMID: 25540354 [PubMed] Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25540354
[h=1]Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells.[/h] Mishel P[SUP]1[/SUP], Bychkov D[SUP]1[/SUP], Kallio-Kokko H[SUP]2[/SUP], Valkonen M[SUP]3[/SUP], Kantele A[SUP]3[/SUP], Mattila P[SUP]1[/SUP], Almusa H[SUP]1[/SUP], Jalovaara P[SUP]1[/SUP], Kainov D[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Here, we sequenced the genome of the influenza A/Finland/741 M/2014(H1N1) virus and found that the virus accumulated oseltamivir resistance mutation H275Y in its neuraminidase during propagation in cell culture. This indicates that propagation in cell culture modifies virus genomes. The instability of influenza genomes should be taken into consideration during drug-sensitivity studies.
Copyright ? 2014 Mishel et al.
PMID: 25540354 [PubMed] Free full text
http://www.ncbi.nlm.nih.gov/pubmed/25540354