tetano
Editor, Senior Moderator
Int Immunopharmacol
. 2021 Mar 10;95:107567.
doi: 10.1016/j.intimp.2021.107567. Online ahead of print.
Landscape of SARS-CoV-2 spike protein-interacting cells in human tissues
Bingfeng Zheng[SUP] 1 [/SUP], Manman Yuan[SUP] 1 [/SUP], Qinhai Ma[SUP] 2 [/SUP], Shenglan Wang[SUP] 1 [/SUP], Yang Tan[SUP] 1 [/SUP], Yizhu Xu[SUP] 1 [/SUP], Jing Ye[SUP] 1 [/SUP], Yanjie Gao[SUP] 1 [/SUP], Xueqing Sun[SUP] 1 [/SUP], Zifeng Yang[SUP] 2 [/SUP], Peipei Xu[SUP] 3 [/SUP], Lingdong Kong[SUP] 1 [/SUP], Xingxin Wu[SUP] 4 [/SUP], Qiang Xu[SUP] 5 [/SUP]
Affiliations
Abstract
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic. However, the mechanism of tissue tropism of SARS-CoV-2 remains unclear. Here, recombinant receptor-binding subdomain 1 of spike protein of SARS-CoV-2 (RBD-SD1) was used as a probe to investigate the potential tropism of SARS-CoV-2 in thirty-three types of normal human tissues. RBD-SD1 probe was observed to interact with cells in reported SARS-CoV-2 infected organs. Interestingly, the RBD-SD1 probe strongly interacted with bone marrow cells in an angiotensin-converting enzyme 2 (ACE2)-independent manner. In addition, SARS-CoV-2 induced the ACE2 mRNA expression in human primary bone marrow cells, suggesting human bone marrow cells may be sensitive to SARS-CoV-2 infection. Therefore, human bone marrow cells could be strongly infected by SARS-CoV-2, which may play an important role in the pathogenesis of COVID-19. These findings provide a deeper understanding of SARS-CoV-2 infection routes, thus contributing to the treatment of COVID-19.
Keywords: Bone marrow cells; Landscape; RBD-SD1 protein probe; SARS-CoV-2.
. 2021 Mar 10;95:107567.
doi: 10.1016/j.intimp.2021.107567. Online ahead of print.
Landscape of SARS-CoV-2 spike protein-interacting cells in human tissues
Bingfeng Zheng[SUP] 1 [/SUP], Manman Yuan[SUP] 1 [/SUP], Qinhai Ma[SUP] 2 [/SUP], Shenglan Wang[SUP] 1 [/SUP], Yang Tan[SUP] 1 [/SUP], Yizhu Xu[SUP] 1 [/SUP], Jing Ye[SUP] 1 [/SUP], Yanjie Gao[SUP] 1 [/SUP], Xueqing Sun[SUP] 1 [/SUP], Zifeng Yang[SUP] 2 [/SUP], Peipei Xu[SUP] 3 [/SUP], Lingdong Kong[SUP] 1 [/SUP], Xingxin Wu[SUP] 4 [/SUP], Qiang Xu[SUP] 5 [/SUP]
Affiliations
- PMID: 33756225
- PMCID: PMC7945790
- DOI: 10.1016/j.intimp.2021.107567
Abstract
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic. However, the mechanism of tissue tropism of SARS-CoV-2 remains unclear. Here, recombinant receptor-binding subdomain 1 of spike protein of SARS-CoV-2 (RBD-SD1) was used as a probe to investigate the potential tropism of SARS-CoV-2 in thirty-three types of normal human tissues. RBD-SD1 probe was observed to interact with cells in reported SARS-CoV-2 infected organs. Interestingly, the RBD-SD1 probe strongly interacted with bone marrow cells in an angiotensin-converting enzyme 2 (ACE2)-independent manner. In addition, SARS-CoV-2 induced the ACE2 mRNA expression in human primary bone marrow cells, suggesting human bone marrow cells may be sensitive to SARS-CoV-2 infection. Therefore, human bone marrow cells could be strongly infected by SARS-CoV-2, which may play an important role in the pathogenesis of COVID-19. These findings provide a deeper understanding of SARS-CoV-2 infection routes, thus contributing to the treatment of COVID-19.
Keywords: Bone marrow cells; Landscape; RBD-SD1 protein probe; SARS-CoV-2.