tetano
Editor, Senior Moderator
Mol Cell Proteomics. 2016 Mar 16. pii: mcp.M116.058016. [Epub ahead of print]
[h=1]Integrated omics and computational glycobiology reveal structural basis for Influenza A virus glycan microheterogeneity and host interactions.[/h] Khatri K[SUP]1[/SUP], Klein JA[SUP]1[/SUP], White MR[SUP]1[/SUP], Grant OC[SUP]2[/SUP], Leymarie N[SUP]1[/SUP], Woods RJ[SUP]2[/SUP], Hartshorn KL[SUP]1[/SUP], Zaia J[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Despite sustained biomedical research effort, influenza A virus remains an imminent threat to the world population and a major healthcare burden. The challenge in developing vaccines against influenza is the ability of the virus to mutate rapidly in response to selective immune pressure. Hemagglutinin is the predominant surface glycoprotein and the primary determinant of antigenicity, virulence and zoonotic potential. Mutations leading to changes in the number of HA glycosylation sites are often reported. Such genetic sequencing studies predict at best the disruption or creation of sequons for N-linked glycosylation; they do not reflect actual phenotypic changes in HA structure. Therefore, combined analysis of glycan micro- and macro-heterogeneity and bioassays will better define the relationships among glycosylation, viral bioactivity and evolution. We present a study that integrates proteomics, glycomics and glycoproteomics of HA before and after adaptation to innate immune system pressure. We combined this information with glycan array and immune lectin binding data to correlate the phenotypic changes with biological activity. Underprocessed glycoforms predominated at the glycosylation sites found to be involved in viral evolution in response to selection pressures and interactions with innate immune-lectins. To understand the structural basis for site-specific glycan microheterogeneity at these sites, we performed structural modeling and molecular dynamics simulations. We observed that the presence of immature, high-mannose type glycans at a particular site correlated with reduced accessibility to glycan remodeling enzymes. Further, the high mannose glycans at sites implicated in immune lectin recognition were predicted to be capable of forming trimeric interactions with the immune-lectin surfactant protein-D.
Copyright ? 2016, The American Society for Biochemistry and Molecular Biology.
[h=4]KEYWORDS:[/h] Glycoprotein Structure*; Glycoproteomics; Glycosylation; Infectious disease; Mass Spectrometry; Viruses
PMID: 26984886 [PubMed - as supplied by publisher] Free full text
[h=1]Integrated omics and computational glycobiology reveal structural basis for Influenza A virus glycan microheterogeneity and host interactions.[/h] Khatri K[SUP]1[/SUP], Klein JA[SUP]1[/SUP], White MR[SUP]1[/SUP], Grant OC[SUP]2[/SUP], Leymarie N[SUP]1[/SUP], Woods RJ[SUP]2[/SUP], Hartshorn KL[SUP]1[/SUP], Zaia J[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Despite sustained biomedical research effort, influenza A virus remains an imminent threat to the world population and a major healthcare burden. The challenge in developing vaccines against influenza is the ability of the virus to mutate rapidly in response to selective immune pressure. Hemagglutinin is the predominant surface glycoprotein and the primary determinant of antigenicity, virulence and zoonotic potential. Mutations leading to changes in the number of HA glycosylation sites are often reported. Such genetic sequencing studies predict at best the disruption or creation of sequons for N-linked glycosylation; they do not reflect actual phenotypic changes in HA structure. Therefore, combined analysis of glycan micro- and macro-heterogeneity and bioassays will better define the relationships among glycosylation, viral bioactivity and evolution. We present a study that integrates proteomics, glycomics and glycoproteomics of HA before and after adaptation to innate immune system pressure. We combined this information with glycan array and immune lectin binding data to correlate the phenotypic changes with biological activity. Underprocessed glycoforms predominated at the glycosylation sites found to be involved in viral evolution in response to selection pressures and interactions with innate immune-lectins. To understand the structural basis for site-specific glycan microheterogeneity at these sites, we performed structural modeling and molecular dynamics simulations. We observed that the presence of immature, high-mannose type glycans at a particular site correlated with reduced accessibility to glycan remodeling enzymes. Further, the high mannose glycans at sites implicated in immune lectin recognition were predicted to be capable of forming trimeric interactions with the immune-lectin surfactant protein-D.
Copyright ? 2016, The American Society for Biochemistry and Molecular Biology.
[h=4]KEYWORDS:[/h] Glycoprotein Structure*; Glycoproteomics; Glycosylation; Infectious disease; Mass Spectrometry; Viruses
PMID: 26984886 [PubMed - as supplied by publisher] Free full text