tetano
Editor, Senior Moderator
Bioorg Med Chem Lett. 2015 Mar 21. pii: S0960-894X(15)00242-5. doi: 10.1016/j.bmcl.2015.03.036. [Epub ahead of print]
[h=1]Integration of antibody by surface functionalization of graphite-encapsulated magnetic beads using ammonia gas plasma technology for capturing influenza A virus.[/h] Sakudo A[SUP]1[/SUP], Chou H[SUP]2[/SUP], Ikuta K[SUP]3[/SUP], Nagatsu M[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antibody-integrated magnetic beads have been functionalized for influenza A virus capture. First, ammonia plasma produced by a radio frequency power source was reacted with the surface of graphite-encapsulated magnetic beads to introduce amino groups. Anti-influenza A virus hemagglutinin antibody was then anchored by its surface sulfide groups to the amino groups on the beads via N-succinimidyl 3-(2-pyridyldithio) propionate. After incubation with influenza A virus, adsorption of the virus to the beads was confirmed by immunochromatography, polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and inoculation of chicken embryonated eggs, indicating that virus infectivity is maintained and that the proposed method is useful for the enhanced detection and isolation of influenza A virus.
Copyright ? 2015 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Amino group; Capture; Gas plasma; Influenza virus; Magnetic beads
PMID: 25857943 [PubMed - as supplied by publisher]
[h=1]Integration of antibody by surface functionalization of graphite-encapsulated magnetic beads using ammonia gas plasma technology for capturing influenza A virus.[/h] Sakudo A[SUP]1[/SUP], Chou H[SUP]2[/SUP], Ikuta K[SUP]3[/SUP], Nagatsu M[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antibody-integrated magnetic beads have been functionalized for influenza A virus capture. First, ammonia plasma produced by a radio frequency power source was reacted with the surface of graphite-encapsulated magnetic beads to introduce amino groups. Anti-influenza A virus hemagglutinin antibody was then anchored by its surface sulfide groups to the amino groups on the beads via N-succinimidyl 3-(2-pyridyldithio) propionate. After incubation with influenza A virus, adsorption of the virus to the beads was confirmed by immunochromatography, polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), and inoculation of chicken embryonated eggs, indicating that virus infectivity is maintained and that the proposed method is useful for the enhanced detection and isolation of influenza A virus.
Copyright ? 2015 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Amino group; Capture; Gas plasma; Influenza virus; Magnetic beads
PMID: 25857943 [PubMed - as supplied by publisher]