tetano
Editor, Senior Moderator
iScience
. 2022 Sep 6;25(10):105085.
doi: 10.1016/j.isci.2022.105085. eCollection 2022 Oct 21.
Assessment of Fcγ receptor-dependent binding of influenza hemagglutinin vaccine-induced antibodies in a non-human primate model
Yuji Masuta[SUP] 1 2 [/SUP], Shokichi Takahama[SUP] 1 [/SUP], Takuto Nogimori[SUP] 1 [/SUP], Saya Moriyama[SUP] 3 [/SUP], Yoshimasa Takahashi[SUP] 3 [/SUP], Takuya Yamamoto[SUP] 1 2 4 5 [/SUP]
Affiliations
Abstract
Several cross-protective antibodies that recognize a broad range of influenza A virus (IAV) strains are known to have functions in virus elimination such as Fcγ receptor (FcγR)-effector function and neutralizing activity against the head region. Although few studies have used primary cells as effector cells, the FcγR-effector function was evaluated after isolating each cell subset. Herein, we established an original assay system to evaluate purified FI6 IgG-mediated binding to hemagglutinin (HA)-expressing cells by flow cytometry using peripheral blood mononuclear cells from cynomolgus macaques. In addition, we evaluated the FcγR-effector function of IAV vaccine-induced anti-HA antibodies in cynomolgus macaques after administering the split vaccine. We found several cell types, mainly classical monocytes, bound to HA-expressing target cells in an FcγR-dependent manner, that were dominant in the binding of the cell population. Thus, this assay system could facilitate the development of a universal influenza vaccine.
Keywords: Biological sciences; Immunological methods; Immunology.
. 2022 Sep 6;25(10):105085.
doi: 10.1016/j.isci.2022.105085. eCollection 2022 Oct 21.
Assessment of Fcγ receptor-dependent binding of influenza hemagglutinin vaccine-induced antibodies in a non-human primate model
Yuji Masuta[SUP] 1 2 [/SUP], Shokichi Takahama[SUP] 1 [/SUP], Takuto Nogimori[SUP] 1 [/SUP], Saya Moriyama[SUP] 3 [/SUP], Yoshimasa Takahashi[SUP] 3 [/SUP], Takuya Yamamoto[SUP] 1 2 4 5 [/SUP]
Affiliations
- PMID: 36147947
- PMCID: PMC9486051
- DOI: 10.1016/j.isci.2022.105085
Abstract
Several cross-protective antibodies that recognize a broad range of influenza A virus (IAV) strains are known to have functions in virus elimination such as Fcγ receptor (FcγR)-effector function and neutralizing activity against the head region. Although few studies have used primary cells as effector cells, the FcγR-effector function was evaluated after isolating each cell subset. Herein, we established an original assay system to evaluate purified FI6 IgG-mediated binding to hemagglutinin (HA)-expressing cells by flow cytometry using peripheral blood mononuclear cells from cynomolgus macaques. In addition, we evaluated the FcγR-effector function of IAV vaccine-induced anti-HA antibodies in cynomolgus macaques after administering the split vaccine. We found several cell types, mainly classical monocytes, bound to HA-expressing target cells in an FcγR-dependent manner, that were dominant in the binding of the cell population. Thus, this assay system could facilitate the development of a universal influenza vaccine.
Keywords: Biological sciences; Immunological methods; Immunology.