tetano
Editor, Senior Moderator
iScience
. 2024 Feb 2;27(3):109100.
doi: 10.1016/j.isci.2024.109100. eCollection 2024 Mar 15. Influenza A virus propagation requires the activation of the unfolded protein response and the accumulation of insoluble protein aggregates
Mariana Marques[SUP] 1 [/SUP], Bruno Ramos[SUP] 1 [/SUP], Hélio Albuquerque[SUP] 2 [/SUP], Marisa Pereira[SUP] 1 [/SUP], Diana Roberta Ribeiro[SUP] 1 [/SUP], Alexandre Nunes[SUP] 1 [/SUP], Jéssica Sarabando[SUP] 1 [/SUP], Daniela Brás[SUP] 3 [/SUP], Ana Rita Ferreira[SUP] 1 [/SUP], Rui Vitorino[SUP] 1 [/SUP], Maria João Amorim[SUP] 3 4 [/SUP], Artur M S Silva[SUP] 2 [/SUP], Ana Raquel Soares[SUP] 1 [/SUP], Daniela Ribeiro[SUP] 1 [/SUP]
Affiliations
Influenza A virus (IAV) employs multiple strategies to manipulate cellular mechanisms and support proper virion formation and propagation. In this study, we performed a detailed analysis of the interplay between IAV and the host cells' proteostasis throughout the entire infectious cycle. We reveal that IAV infection activates the inositol requiring enzyme 1 (IRE1) branch of the unfolded protein response, and that this activation is important for an efficient infection. We further observed the accumulation of virus-induced insoluble protein aggregates, containing both viral and host proteins, associated with a dysregulation of the host cell RNA metabolism. Our data indicate that this accumulation is important for IAV propagation and favors the final steps of the infection cycle, more specifically the virion assembly. These findings reveal additional mechanisms by which IAV disrupts host proteostasis and uncovers new cellular targets that can be explored for the development of host-directed antiviral strategies.
. 2024 Feb 2;27(3):109100.
doi: 10.1016/j.isci.2024.109100. eCollection 2024 Mar 15. Influenza A virus propagation requires the activation of the unfolded protein response and the accumulation of insoluble protein aggregates
Mariana Marques[SUP] 1 [/SUP], Bruno Ramos[SUP] 1 [/SUP], Hélio Albuquerque[SUP] 2 [/SUP], Marisa Pereira[SUP] 1 [/SUP], Diana Roberta Ribeiro[SUP] 1 [/SUP], Alexandre Nunes[SUP] 1 [/SUP], Jéssica Sarabando[SUP] 1 [/SUP], Daniela Brás[SUP] 3 [/SUP], Ana Rita Ferreira[SUP] 1 [/SUP], Rui Vitorino[SUP] 1 [/SUP], Maria João Amorim[SUP] 3 4 [/SUP], Artur M S Silva[SUP] 2 [/SUP], Ana Raquel Soares[SUP] 1 [/SUP], Daniela Ribeiro[SUP] 1 [/SUP]
Affiliations
- PMID: 38405606
- PMCID: PMC10884513
- DOI: 10.1016/j.isci.2024.109100
Influenza A virus (IAV) employs multiple strategies to manipulate cellular mechanisms and support proper virion formation and propagation. In this study, we performed a detailed analysis of the interplay between IAV and the host cells' proteostasis throughout the entire infectious cycle. We reveal that IAV infection activates the inositol requiring enzyme 1 (IRE1) branch of the unfolded protein response, and that this activation is important for an efficient infection. We further observed the accumulation of virus-induced insoluble protein aggregates, containing both viral and host proteins, associated with a dysregulation of the host cell RNA metabolism. Our data indicate that this accumulation is important for IAV propagation and favors the final steps of the infection cycle, more specifically the virion assembly. These findings reveal additional mechanisms by which IAV disrupts host proteostasis and uncovers new cellular targets that can be explored for the development of host-directed antiviral strategies.