tetano
Editor, Senior Moderator
Med Microbiol Immunol. 2016 Dec 20. doi: 10.1007/s00430-016-0489-3. [Epub ahead of print]
[h=1]iTRAQ-based quantitative proteomics reveals important host factors involved in the high pathogenicity of the H5N1 avian influenza virus in mice.[/h] Hu J[SUP]1,[/SUP][SUP]2[/SUP], Gao Z[SUP]1,[/SUP][SUP]2[/SUP], Wang X[SUP]1,[/SUP][SUP]2[/SUP], Gu M[SUP]1,[/SUP][SUP]2[/SUP], Liang Y[SUP]1,[/SUP][SUP]2[/SUP], Liu X[SUP]1,[/SUP][SUP]2[/SUP], Hu S[SUP]1,[/SUP][SUP]2[/SUP], Liu H[SUP]1,[/SUP][SUP]2[/SUP], Liu W[SUP]1,[/SUP][SUP]2[/SUP], Chen S[SUP]1,[/SUP][SUP]2[/SUP], Peng D[SUP]1,[/SUP][SUP]2[/SUP], Liu X[SUP]3,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We previously reported a pair of H5N1 avian influenza viruses which are genetically similar but differ greatly in their virulence in mice. A/Chicken/Jiangsu/k0402/2010 (CK10) is highly lethal to mice, whereas A/Goose/Jiangsu/k0403/2010 (GS10) is avirulent. In this study, to investigate the host factors that account for their virulence discrepancy, we compared the pathology and host proteome of the CK10- or GS10-infected mouse lung. Moderate lung injury was observed from CK10-infected animals as early as the first day of infection, and the pathology steadily progressed at later time point. However, only mild lesions were observed in GS10-infected mouse lung at the late infection stage. Using the quantitative iTRAQ coupled LC-MS/MS method, we first found that more significantly differentially expressed (DE) proteins were stimulated by GS10 compared with CK10. However, bio-function analysis of the DE proteins suggested that CK10 induced much stronger inflammatory response-related functions than GS10. Canonical pathway analysis also demonstrated that CK10 highly activated the "Acute Phase Response Signaling," which results in a wide range of biological activities in response to viral infection, including many inflammatory processes. Further in-depth analysis showed that CK10 exacerbated acute lung injury-associated responses, including inflammatory response, cell death, reactive oxygen species production and complement response. In addition, some of these identified proteins that associated with the lung injury were further confirmed to be regulated in vitro. Therefore, our findings suggest that the early increased lung injury-associated host response induced by CK10 may contribute to the lung pathology and the high virulence of this virus in mice.
[h=4]KEYWORDS:[/h] Highly pathogenic H5N1 virus; Lung injury; Pathogenesis; Proteomics; iTRAQ
PMID: 28000052 DOI: 10.1007/s00430-016-0489-3
[PubMed - as supplied by publisher]
[h=1]iTRAQ-based quantitative proteomics reveals important host factors involved in the high pathogenicity of the H5N1 avian influenza virus in mice.[/h] Hu J[SUP]1,[/SUP][SUP]2[/SUP], Gao Z[SUP]1,[/SUP][SUP]2[/SUP], Wang X[SUP]1,[/SUP][SUP]2[/SUP], Gu M[SUP]1,[/SUP][SUP]2[/SUP], Liang Y[SUP]1,[/SUP][SUP]2[/SUP], Liu X[SUP]1,[/SUP][SUP]2[/SUP], Hu S[SUP]1,[/SUP][SUP]2[/SUP], Liu H[SUP]1,[/SUP][SUP]2[/SUP], Liu W[SUP]1,[/SUP][SUP]2[/SUP], Chen S[SUP]1,[/SUP][SUP]2[/SUP], Peng D[SUP]1,[/SUP][SUP]2[/SUP], Liu X[SUP]3,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] We previously reported a pair of H5N1 avian influenza viruses which are genetically similar but differ greatly in their virulence in mice. A/Chicken/Jiangsu/k0402/2010 (CK10) is highly lethal to mice, whereas A/Goose/Jiangsu/k0403/2010 (GS10) is avirulent. In this study, to investigate the host factors that account for their virulence discrepancy, we compared the pathology and host proteome of the CK10- or GS10-infected mouse lung. Moderate lung injury was observed from CK10-infected animals as early as the first day of infection, and the pathology steadily progressed at later time point. However, only mild lesions were observed in GS10-infected mouse lung at the late infection stage. Using the quantitative iTRAQ coupled LC-MS/MS method, we first found that more significantly differentially expressed (DE) proteins were stimulated by GS10 compared with CK10. However, bio-function analysis of the DE proteins suggested that CK10 induced much stronger inflammatory response-related functions than GS10. Canonical pathway analysis also demonstrated that CK10 highly activated the "Acute Phase Response Signaling," which results in a wide range of biological activities in response to viral infection, including many inflammatory processes. Further in-depth analysis showed that CK10 exacerbated acute lung injury-associated responses, including inflammatory response, cell death, reactive oxygen species production and complement response. In addition, some of these identified proteins that associated with the lung injury were further confirmed to be regulated in vitro. Therefore, our findings suggest that the early increased lung injury-associated host response induced by CK10 may contribute to the lung pathology and the high virulence of this virus in mice.
[h=4]KEYWORDS:[/h] Highly pathogenic H5N1 virus; Lung injury; Pathogenesis; Proteomics; iTRAQ
PMID: 28000052 DOI: 10.1007/s00430-016-0489-3
[PubMed - as supplied by publisher]