tetano
Editor, Senior Moderator
J Clin Microbiol
. 2020 Oct 5;JCM.02148-20.
doi: 10.1128/JCM.02148-20. Online ahead of print.
Application, Verification and Implementation of SARS-CoV-2 Serologic Assays with Emergency Use Authorization
Elitza S Theel[SUP] 1 [/SUP], Marc Roger Couturier[SUP] 2 3 [/SUP], Laura Filkins[SUP] 4 [/SUP], Elizabeth Palavecino[SUP] 5 [/SUP], Stephanie Mitchell[SUP] 6 [/SUP], Sheldon Campbell[SUP] 7 8 [/SUP], Michael Pentella[SUP] 9 10 [/SUP], Susan Butler-Wu[SUP] 11 [/SUP], Kurt Jerke[SUP] 12 [/SUP], Vaishali Dharmarha[SUP] 13 [/SUP], Peggy McNult[SUP] 13 [/SUP], Audrey N Schuetz, American Society for Microbiology Clinical and Public Health Microbiology Committee, Subcommittee on Laboratory Practices
Affiliations
Abstract
Interest continues to grow regarding the role of serologic assays for the detection of prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The U.S. Food and Drug Administration (FDA) has granted Emergency Use Authorization (EUA) status to many SARS-CoV-2 serologic assays. In this document, expert recommendations from clinical microbiologist members of the American Society for Microbiology (ASM) concerning detailed verification strategies for SARS-CoV-2 serologic assays with FDA EUA are provided, as are insights into assay limitations and reporting considerations for laboratories. Assessments concerning single and multi-antibody isotype detection assays, which may provide either differentiated or non-differentiated (i.e., total antibody) antibody class results, are addressed. Additional considerations prior to assay implementation are also discussed, including biosafety, quality control and proficiency testing strategies. As the landscape of SARS-CoV-2 serologic testing is rapidly changing, this document provides updated guidance for laboratorians on application of these assays.
. 2020 Oct 5;JCM.02148-20.
doi: 10.1128/JCM.02148-20. Online ahead of print.
Application, Verification and Implementation of SARS-CoV-2 Serologic Assays with Emergency Use Authorization
Elitza S Theel[SUP] 1 [/SUP], Marc Roger Couturier[SUP] 2 3 [/SUP], Laura Filkins[SUP] 4 [/SUP], Elizabeth Palavecino[SUP] 5 [/SUP], Stephanie Mitchell[SUP] 6 [/SUP], Sheldon Campbell[SUP] 7 8 [/SUP], Michael Pentella[SUP] 9 10 [/SUP], Susan Butler-Wu[SUP] 11 [/SUP], Kurt Jerke[SUP] 12 [/SUP], Vaishali Dharmarha[SUP] 13 [/SUP], Peggy McNult[SUP] 13 [/SUP], Audrey N Schuetz, American Society for Microbiology Clinical and Public Health Microbiology Committee, Subcommittee on Laboratory Practices
Affiliations
- PMID: 33020185
- DOI: 10.1128/JCM.02148-20
Abstract
Interest continues to grow regarding the role of serologic assays for the detection of prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The U.S. Food and Drug Administration (FDA) has granted Emergency Use Authorization (EUA) status to many SARS-CoV-2 serologic assays. In this document, expert recommendations from clinical microbiologist members of the American Society for Microbiology (ASM) concerning detailed verification strategies for SARS-CoV-2 serologic assays with FDA EUA are provided, as are insights into assay limitations and reporting considerations for laboratories. Assessments concerning single and multi-antibody isotype detection assays, which may provide either differentiated or non-differentiated (i.e., total antibody) antibody class results, are addressed. Additional considerations prior to assay implementation are also discussed, including biosafety, quality control and proficiency testing strategies. As the landscape of SARS-CoV-2 serologic testing is rapidly changing, this document provides updated guidance for laboratorians on application of these assays.