tetano
Editor, Senior Moderator
J Clin Virol
. 2020 Dec 4;134:104712.
doi: 10.1016/j.jcv.2020.104712. Online ahead of print.
Evaluation of rapid antigen detection kit from the WHO Emergency Use List for detecting SARS-CoV-2
Gannon C K Mak[SUP] 1 [/SUP], Stephen S Y Lau[SUP] 2 [/SUP], Kitty K Y Wong[SUP] 2 [/SUP], Nancy L S Chow[SUP] 2 [/SUP], C S Lau[SUP] 2 [/SUP], Edman T K Lam[SUP] 2 [/SUP], Rickjason C W Chan[SUP] 2 [/SUP], Dominic N C Tsang[SUP] 2 [/SUP]
Affiliations
Abstract
Background: Currently, there are two rapid antigen detection (RAD) kits from the WHO Emergency Use List for detecting SARS-CoV-2.
Objective: The Panbio COVID-19 Ag Rapid Test Device was selected to evaluate the performance for detecting SARS-CoV-2.
Study design: Analytical sensitivity for the detection of SARS-CoV-2 virus was determined by limit of detection (LOD) using RT-PCR as a reference method. Clinical sensitivity was evaluated by using respiratory specimens collected from confirmed COVID-19 patients.
Results: The LOD results showed that the RAD kit was 100 fold less sensitive than RT-PCR. Clinical sensitivity of the RAD kit was 68.6 % for detecting specimens from COVID-19 patients.
Conclusions: The RAD kit evaluated in the present study shared similar performance with another kit from the WHO Emergency Use List, the Standard Q COVID-19 Ag. Understanding the clinical characteristics of RAD kits can guide us to decide different testing strategies in different settings.
Keywords: 2019 novel coronavirus; COVID-19; RT-PCR; Rapid antigen detection; SARS-CoV-2.
. 2020 Dec 4;134:104712.
doi: 10.1016/j.jcv.2020.104712. Online ahead of print.
Evaluation of rapid antigen detection kit from the WHO Emergency Use List for detecting SARS-CoV-2
Gannon C K Mak[SUP] 1 [/SUP], Stephen S Y Lau[SUP] 2 [/SUP], Kitty K Y Wong[SUP] 2 [/SUP], Nancy L S Chow[SUP] 2 [/SUP], C S Lau[SUP] 2 [/SUP], Edman T K Lam[SUP] 2 [/SUP], Rickjason C W Chan[SUP] 2 [/SUP], Dominic N C Tsang[SUP] 2 [/SUP]
Affiliations
- PMID: 33338894
- PMCID: PMC7716730
- DOI: 10.1016/j.jcv.2020.104712
Abstract
Background: Currently, there are two rapid antigen detection (RAD) kits from the WHO Emergency Use List for detecting SARS-CoV-2.
Objective: The Panbio COVID-19 Ag Rapid Test Device was selected to evaluate the performance for detecting SARS-CoV-2.
Study design: Analytical sensitivity for the detection of SARS-CoV-2 virus was determined by limit of detection (LOD) using RT-PCR as a reference method. Clinical sensitivity was evaluated by using respiratory specimens collected from confirmed COVID-19 patients.
Results: The LOD results showed that the RAD kit was 100 fold less sensitive than RT-PCR. Clinical sensitivity of the RAD kit was 68.6 % for detecting specimens from COVID-19 patients.
Conclusions: The RAD kit evaluated in the present study shared similar performance with another kit from the WHO Emergency Use List, the Standard Q COVID-19 Ag. Understanding the clinical characteristics of RAD kits can guide us to decide different testing strategies in different settings.
Keywords: 2019 novel coronavirus; COVID-19; RT-PCR; Rapid antigen detection; SARS-CoV-2.