tetano
Editor, Senior Moderator
J Clin Virol Plus
. 2021 Dec;1(4):100042.
doi: 10.1016/j.jcvp.2021.100042. Epub 2021 Sep 24.
Multi-center evaluation of Cepheid Xpert® Xpress SARS-CoV-2/Flu/RSV molecular point-of-care test
John Sluimer[SUP] 1 [/SUP], Gabriel Goderski[SUP] 1 [/SUP], Sharon van den Brink[SUP] 1 [/SUP], Maaike Broeders[SUP] 2 [/SUP], Janette Rahamat-Langendoen[SUP] 3 [/SUP], Euníce Then[SUP] 1 [/SUP], Lisa Wijsman[SUP] 1 [/SUP], Femke Wolters[SUP] 3 [/SUP], Jeroen van de Bovenkamp[SUP] 2 [/SUP], Willem Jg Melchers[SUP] 3 [/SUP], Adam Meijer[SUP] 1 [/SUP]
Affiliations
Abstract
Background: SARS-CoV-2 is taking a huge toll on society while influenza and RSV detection are also becoming more important. These viruses pose a high burden on health care. Rapid and accurate diagnostics for these pathogens are important for swift triage in the hospital. Fast molecular point of care test (mPOCT) assays for these pathogens can prove an alternative. Here a multi-center evaluation of the Xpert® Xpress SARS-CoV-2/Flu/RSV assay is reported.
Study design: The Xpert® Xpress SARS-CoV-2/Flu/RSV assay was compared to three reference assays at three Dutch medical microbiology laboratories. An external quality assessment panel consisting of 16 specimens containing SARS-CoV-2, influenza viruses, RSV or human seasonal coronaviruses, or a combination thereof were used. Clinical specimens containing SARS-CoV-2 (n = 57), influenza viruses (n = 21) or RSV (n = 12), at a wide range of relevant concentrations were used. One laboratory also tested zoonotic avian and swine influenza viruses, and eight relevant SARS-CoV-2 variants.
Results: The Xpert® Xpress SARS-CoV-2/Flu/RSV assay showed equal performance compared to the reference assays. All SARS-CoV-2 variants of interest and variants of concern were accurately detected. Human seasonal coronaviruses were not detected. All four circulating seasonal influenza virus subtypes/lineages and both RSV types were accurately detected as well as a set of recent zoonotic avian and swine influenza viruses. The clinical specimens showed 98.2% concordance using this assay.
Conclusion: The Xpert® Xpress SARS-CoV-2/Flu/RSV assay is a good alternative for accurate detection for SARS-CoV-2, influenza type A virus, influenza type B virus and RSV types A and B detection in a short timeframe.
Keywords: COVID-19; Influenza virus; Molecular point-of-care test; RSV; SARS-CoV-2; Xpert Xpress.
. 2021 Dec;1(4):100042.
doi: 10.1016/j.jcvp.2021.100042. Epub 2021 Sep 24.
Multi-center evaluation of Cepheid Xpert® Xpress SARS-CoV-2/Flu/RSV molecular point-of-care test
John Sluimer[SUP] 1 [/SUP], Gabriel Goderski[SUP] 1 [/SUP], Sharon van den Brink[SUP] 1 [/SUP], Maaike Broeders[SUP] 2 [/SUP], Janette Rahamat-Langendoen[SUP] 3 [/SUP], Euníce Then[SUP] 1 [/SUP], Lisa Wijsman[SUP] 1 [/SUP], Femke Wolters[SUP] 3 [/SUP], Jeroen van de Bovenkamp[SUP] 2 [/SUP], Willem Jg Melchers[SUP] 3 [/SUP], Adam Meijer[SUP] 1 [/SUP]
Affiliations
- PMID: 35262024
- PMCID: PMC8461038
- DOI: 10.1016/j.jcvp.2021.100042
Abstract
Background: SARS-CoV-2 is taking a huge toll on society while influenza and RSV detection are also becoming more important. These viruses pose a high burden on health care. Rapid and accurate diagnostics for these pathogens are important for swift triage in the hospital. Fast molecular point of care test (mPOCT) assays for these pathogens can prove an alternative. Here a multi-center evaluation of the Xpert® Xpress SARS-CoV-2/Flu/RSV assay is reported.
Study design: The Xpert® Xpress SARS-CoV-2/Flu/RSV assay was compared to three reference assays at three Dutch medical microbiology laboratories. An external quality assessment panel consisting of 16 specimens containing SARS-CoV-2, influenza viruses, RSV or human seasonal coronaviruses, or a combination thereof were used. Clinical specimens containing SARS-CoV-2 (n = 57), influenza viruses (n = 21) or RSV (n = 12), at a wide range of relevant concentrations were used. One laboratory also tested zoonotic avian and swine influenza viruses, and eight relevant SARS-CoV-2 variants.
Results: The Xpert® Xpress SARS-CoV-2/Flu/RSV assay showed equal performance compared to the reference assays. All SARS-CoV-2 variants of interest and variants of concern were accurately detected. Human seasonal coronaviruses were not detected. All four circulating seasonal influenza virus subtypes/lineages and both RSV types were accurately detected as well as a set of recent zoonotic avian and swine influenza viruses. The clinical specimens showed 98.2% concordance using this assay.
Conclusion: The Xpert® Xpress SARS-CoV-2/Flu/RSV assay is a good alternative for accurate detection for SARS-CoV-2, influenza type A virus, influenza type B virus and RSV types A and B detection in a short timeframe.
Keywords: COVID-19; Influenza virus; Molecular point-of-care test; RSV; SARS-CoV-2; Xpert Xpress.