Giuseppe
Emeritus
[Source: Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]
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Giant Blood Marseillevirus recovered from asymptomatic blood donors
Nikolay Popgeorgiev 1, Micka?l Boyer 1,2, Laura Fancello 1, Sonia Monteil 1, Catherine Robert 1, Romain Rivet 1, Claude Nappez 1, Said Azza 1, Jacques Chiaroni 3, Didier Raoult 1 and Christelle Desnues 1,*
Author Affiliations: <SUP>1</SUP>Aix Marseille Universit?, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, 13005 Marseille, France <SUP>2</SUP>Current address: Danone Research, 92190 Meudon, France <SUP>3</SUP>Aix-Marseille Universit?, Etablissement Fran?ais du Sang, Anthropologie bio-culturelle, CNRS UMR6578, 13005 Marseille, France
*Corresponding author: Christelle Desnues, Unit? de recherche sur les maladies infectieuses et tropicales ?mergentes, URMITE CNRS-IRD UMR 7278, Aix-Marseille Universit?, Facult? de m?decine, 27 Bd Jean Moulin, Marseille 13385, France. E-mail: christelle.desnues@univ-amu.fr, Phone: 00 33 4 91 32 46 30
Abstract
The study of the human virome is still in its infancy, especially with regards to the viral content of the blood of people that are apparently disease free. In this study, the genome of a new giant virus that is related to the amoeba-infecting pathogen Marseillevirus was recovered from blood donors using high-throughput sequencing. Viral antigens were identified by an immunoconversion assay. The virus was visualized with transmission electron microscopy and fluorescence in situ hybridization and was grown in human T-cell lymphocytes. Specific antibody reactions were used to identify viral proteins in the PCR-positive blood donor. Finally, we tested 20 additional blood donors. Three had antibodies directed against this virus and two had circulating viral DNA. This study shows that giant viruses, which are missed by the use of ultrafilters, are part of the human blood viromes. The putative pathogenic role of giant viruses in humans remains undefined.
Received February 5, 2013. Revision received March 19, 2013. Accepted March 21, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
-Nikolay Popgeorgiev 1, Micka?l Boyer 1,2, Laura Fancello 1, Sonia Monteil 1, Catherine Robert 1, Romain Rivet 1, Claude Nappez 1, Said Azza 1, Jacques Chiaroni 3, Didier Raoult 1 and Christelle Desnues 1,*
Author Affiliations: <SUP>1</SUP>Aix Marseille Universit?, URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, 13005 Marseille, France <SUP>2</SUP>Current address: Danone Research, 92190 Meudon, France <SUP>3</SUP>Aix-Marseille Universit?, Etablissement Fran?ais du Sang, Anthropologie bio-culturelle, CNRS UMR6578, 13005 Marseille, France
*Corresponding author: Christelle Desnues, Unit? de recherche sur les maladies infectieuses et tropicales ?mergentes, URMITE CNRS-IRD UMR 7278, Aix-Marseille Universit?, Facult? de m?decine, 27 Bd Jean Moulin, Marseille 13385, France. E-mail: christelle.desnues@univ-amu.fr, Phone: 00 33 4 91 32 46 30
Abstract
The study of the human virome is still in its infancy, especially with regards to the viral content of the blood of people that are apparently disease free. In this study, the genome of a new giant virus that is related to the amoeba-infecting pathogen Marseillevirus was recovered from blood donors using high-throughput sequencing. Viral antigens were identified by an immunoconversion assay. The virus was visualized with transmission electron microscopy and fluorescence in situ hybridization and was grown in human T-cell lymphocytes. Specific antibody reactions were used to identify viral proteins in the PCR-positive blood donor. Finally, we tested 20 additional blood donors. Three had antibodies directed against this virus and two had circulating viral DNA. This study shows that giant viruses, which are missed by the use of ultrafilters, are part of the human blood viromes. The putative pathogenic role of giant viruses in humans remains undefined.
Received February 5, 2013. Revision received March 19, 2013. Accepted March 21, 2013.
? The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
For Permissions, please e-mail: journals.permissions@oup.com.
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