tetano
Editor, Senior Moderator
J Med Biochem
. 2023 Oct 27;42(4):658-664.
doi: 10.5937/jomb0-43822. Evaluation of a new molecular test for the detection of SARS-CoV-2 nucleic acid in salivary samples
Ilaria Talli[SUP] 1 [/SUP], Andrea Padoan[SUP] 1 [/SUP], Stefania Moz[SUP] 1 [/SUP], Filippo Navaglia[SUP] 1 [/SUP], Mario Plebani[SUP] 1 [/SUP], Daniela Basso[SUP] 1 [/SUP]
Affiliations
in English, Serbian
Background: Molecular testing is considered the gold standard for the detection of SARS-CoV-2. This study aimed to compare the performance of the P742H SARS-CoV-2 Nucleic Acid Multiplex Detection Kit in salivary samples, with respect to the 732HF Novel Coronavirus (2019-nCoV) Nucleic Acid Detection Kit and the TaqPath COVID-19 CEIVD RT-PCR Kit, used at University-Hospital of Padova, Italy.
Methods: One hundred twenty-four salivary samples selfcollected by healthcare workers (HCW) during the screening program at University-Hospital of Padova, Italy, from Oct to Nov 2022, were included in the study. RNA extraction was performed by Viral DNA and RNA Extraction Kit (Technogenetics, Lodi, Italy) and amplification by P742H and 732HF (Technogenetics, Lodi, Italy). RNA was extracted using MagNa Pure 96 DNA and Viral NA Small Volume Kit (Roche, Switzerland) for TaqPath analysis (Thermo Fisher Scientific, USA).
Keywords: COVID-19; SARS-CoV-2; molecular testing; nucleic acid amplification; salivary samples.
. 2023 Oct 27;42(4):658-664.
doi: 10.5937/jomb0-43822. Evaluation of a new molecular test for the detection of SARS-CoV-2 nucleic acid in salivary samples
Ilaria Talli[SUP] 1 [/SUP], Andrea Padoan[SUP] 1 [/SUP], Stefania Moz[SUP] 1 [/SUP], Filippo Navaglia[SUP] 1 [/SUP], Mario Plebani[SUP] 1 [/SUP], Daniela Basso[SUP] 1 [/SUP]
Affiliations
- PMID: 38084251
- PMCID: PMC10710785
- DOI: 10.5937/jomb0-43822
in English, Serbian
Background: Molecular testing is considered the gold standard for the detection of SARS-CoV-2. This study aimed to compare the performance of the P742H SARS-CoV-2 Nucleic Acid Multiplex Detection Kit in salivary samples, with respect to the 732HF Novel Coronavirus (2019-nCoV) Nucleic Acid Detection Kit and the TaqPath COVID-19 CEIVD RT-PCR Kit, used at University-Hospital of Padova, Italy.
Methods: One hundred twenty-four salivary samples selfcollected by healthcare workers (HCW) during the screening program at University-Hospital of Padova, Italy, from Oct to Nov 2022, were included in the study. RNA extraction was performed by Viral DNA and RNA Extraction Kit (Technogenetics, Lodi, Italy) and amplification by P742H and 732HF (Technogenetics, Lodi, Italy). RNA was extracted using MagNa Pure 96 DNA and Viral NA Small Volume Kit (Roche, Switzerland) for TaqPath analysis (Thermo Fisher Scientific, USA).
Keywords: COVID-19; SARS-CoV-2; molecular testing; nucleic acid amplification; salivary samples.