tetano
Editor, Senior Moderator
J Med Virol
. 2020 Aug 25.
doi: 10.1002/jmv.26460. Online ahead of print.
Saliva sample pooling for the detection of SARS-CoV-2
Ekawat Pasomsub[SUP] 1 [/SUP], Siriorn P Watcharananan[SUP] 2 [/SUP], Treewat Watthanachockchai[SUP] 1 [/SUP], Kingkan Rakmanee[SUP] 1 [/SUP], Boonrat Tassaneetrithep[SUP] 3 [/SUP], Sasisopin Kiertiburanakul[SUP] 2 [/SUP], Angsana Phuphuakrat[SUP] 2 [/SUP]
Affiliations
Abstract
As the battle against coronavirus disease 2019 pandemic continues, an increase in workload and medical expenses have been a concern to the health care system worldwide. Developing a measure that helps to conserve the health care resource is, therefore, highly desirable, and the pooling of the specimens for testing is one of the attractive strategies. Recently we showed that saliva could be a potential alternative specimen for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by real-time polymerase chain reaction (RT-PCR). In the present study, we performed the pooling of saliva specimens for testing by SARS-CoV-2 RT-PCR. We showed that the saliva pool of either five or ten samples, by allowing the detection of either gene in the pool at an increased cycle threshold cut-off value, further performing individual sample testing in the positive pools did not compromise the detection of SARS-CoV-2. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; RT-PCR; SARS-CoV-2; pooling; saliva.
. 2020 Aug 25.
doi: 10.1002/jmv.26460. Online ahead of print.
Saliva sample pooling for the detection of SARS-CoV-2
Ekawat Pasomsub[SUP] 1 [/SUP], Siriorn P Watcharananan[SUP] 2 [/SUP], Treewat Watthanachockchai[SUP] 1 [/SUP], Kingkan Rakmanee[SUP] 1 [/SUP], Boonrat Tassaneetrithep[SUP] 3 [/SUP], Sasisopin Kiertiburanakul[SUP] 2 [/SUP], Angsana Phuphuakrat[SUP] 2 [/SUP]
Affiliations
- PMID: 32841429
- DOI: 10.1002/jmv.26460
Abstract
As the battle against coronavirus disease 2019 pandemic continues, an increase in workload and medical expenses have been a concern to the health care system worldwide. Developing a measure that helps to conserve the health care resource is, therefore, highly desirable, and the pooling of the specimens for testing is one of the attractive strategies. Recently we showed that saliva could be a potential alternative specimen for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by real-time polymerase chain reaction (RT-PCR). In the present study, we performed the pooling of saliva specimens for testing by SARS-CoV-2 RT-PCR. We showed that the saliva pool of either five or ten samples, by allowing the detection of either gene in the pool at an increased cycle threshold cut-off value, further performing individual sample testing in the positive pools did not compromise the detection of SARS-CoV-2. This article is protected by copyright. All rights reserved.
Keywords: COVID-19; RT-PCR; SARS-CoV-2; pooling; saliva.