tetano
Editor, Senior Moderator
J Virol
. 2022 Jun 2;e0041222.
doi: 10.1128/jvi.00412-22. Online ahead of print.
SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication
Hainan Liu[SUP] #[/SUP][SUP] 1 [/SUP], Yu Bai[SUP] #[/SUP][SUP] 2 [/SUP], Xun Zhang[SUP] 2 [/SUP], Ting Gao[SUP] 1 [/SUP], Yue Liu[SUP] 1 [/SUP], Entao Li[SUP] 3 [/SUP], Xuefeng Wang[SUP] 3 [/SUP], Zheng Cao[SUP] 4 [/SUP], Lin Zhu[SUP] 1 [/SUP], Qincai Dong[SUP] 1 [/SUP], Yong Hu[SUP] 1 [/SUP], Guangfei Wang[SUP] 1 [/SUP], Caiwei Song[SUP] 1 [/SUP], Xiayang Niu[SUP] 2 [/SUP], Tong Zheng[SUP] 1 [/SUP], Di Wang[SUP] 2 [/SUP], Zijing Liu[SUP] 1 [/SUP], Yanwen Jin[SUP] 1 [/SUP], Ping Li[SUP] 1 [/SUP], Xiuwu Bian[SUP] 5 [/SUP], Cheng Cao[SUP] 1 [/SUP], Xuan Liu[SUP] 1 [/SUP]
Affiliations
Abstract
SARS-CoV-2 is the causative agent of the ongoing pandemic of coronavirus disease 2019 (COVID-19) and poses a significant threat to global health. N protein (NP), which is a major pathogenic protein among betacoronaviruses, binds to the viral RNA genome to allow viral genome packaging and viral particle release. Recent studies showed that NP antagonizes interferon (IFN) induction and mediates phase separation. Using live SARS-CoV-2 viruses, this study provides solid evidence showing that SARS-CoV-2 NP associates with G3BP1 and G3BP2 in vitro and in vivo. NP[SUP]SARS-CoV-2[/SUP] could efficiently suppress G3BP-mediated SG formation and potentiate viral infection by overcoming G3BP1-mediated antiviral innate immunity. G3BP1 conditional knockout mice (g3bp1[SUP]fl/fL[/SUP], Sftpc-Cre) exhibit significantly higher lung viral loads after SARS-CoV-2 infection than wild-type mice. Our findings contribute to the growing body of knowledge regarding the pathogenicity of NP[SUP]SARS-CoV-2[/SUP] and provide insight into new therapeutics targeting NP[SUP]SARS-CoV-2[/SUP]. IMPORTANCE In this study, by in vitro assay and live SARS-CoV-2 virus infection, we provide solid evidence that the SARS-CoV-2 NP associates with G3BP1 and G3BP2 in vitro and in vivo. NP[SUP]SARS-CoV-2[/SUP] could efficiently suppress G3BP-mediated SG formation and potentiate viral infection by overcoming antiviral innate immunity mediated by G3BP1 in A549 cell lines and G3BP1 conditional knockout mice (g3bp1-cKO) mice, which provide in-depth evidence showing the mechanism underlying NP-related SARS-CoV-2 pathogenesis through G3BPs.
Keywords: G3BP1; SARS-CoV-2; nucleocapsid protein; stress granule.
. 2022 Jun 2;e0041222.
doi: 10.1128/jvi.00412-22. Online ahead of print.
SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication
Hainan Liu[SUP] #[/SUP][SUP] 1 [/SUP], Yu Bai[SUP] #[/SUP][SUP] 2 [/SUP], Xun Zhang[SUP] 2 [/SUP], Ting Gao[SUP] 1 [/SUP], Yue Liu[SUP] 1 [/SUP], Entao Li[SUP] 3 [/SUP], Xuefeng Wang[SUP] 3 [/SUP], Zheng Cao[SUP] 4 [/SUP], Lin Zhu[SUP] 1 [/SUP], Qincai Dong[SUP] 1 [/SUP], Yong Hu[SUP] 1 [/SUP], Guangfei Wang[SUP] 1 [/SUP], Caiwei Song[SUP] 1 [/SUP], Xiayang Niu[SUP] 2 [/SUP], Tong Zheng[SUP] 1 [/SUP], Di Wang[SUP] 2 [/SUP], Zijing Liu[SUP] 1 [/SUP], Yanwen Jin[SUP] 1 [/SUP], Ping Li[SUP] 1 [/SUP], Xiuwu Bian[SUP] 5 [/SUP], Cheng Cao[SUP] 1 [/SUP], Xuan Liu[SUP] 1 [/SUP]
Affiliations
- PMID: 35652658
- DOI: 10.1128/jvi.00412-22
Abstract
SARS-CoV-2 is the causative agent of the ongoing pandemic of coronavirus disease 2019 (COVID-19) and poses a significant threat to global health. N protein (NP), which is a major pathogenic protein among betacoronaviruses, binds to the viral RNA genome to allow viral genome packaging and viral particle release. Recent studies showed that NP antagonizes interferon (IFN) induction and mediates phase separation. Using live SARS-CoV-2 viruses, this study provides solid evidence showing that SARS-CoV-2 NP associates with G3BP1 and G3BP2 in vitro and in vivo. NP[SUP]SARS-CoV-2[/SUP] could efficiently suppress G3BP-mediated SG formation and potentiate viral infection by overcoming G3BP1-mediated antiviral innate immunity. G3BP1 conditional knockout mice (g3bp1[SUP]fl/fL[/SUP], Sftpc-Cre) exhibit significantly higher lung viral loads after SARS-CoV-2 infection than wild-type mice. Our findings contribute to the growing body of knowledge regarding the pathogenicity of NP[SUP]SARS-CoV-2[/SUP] and provide insight into new therapeutics targeting NP[SUP]SARS-CoV-2[/SUP]. IMPORTANCE In this study, by in vitro assay and live SARS-CoV-2 virus infection, we provide solid evidence that the SARS-CoV-2 NP associates with G3BP1 and G3BP2 in vitro and in vivo. NP[SUP]SARS-CoV-2[/SUP] could efficiently suppress G3BP-mediated SG formation and potentiate viral infection by overcoming antiviral innate immunity mediated by G3BP1 in A549 cell lines and G3BP1 conditional knockout mice (g3bp1-cKO) mice, which provide in-depth evidence showing the mechanism underlying NP-related SARS-CoV-2 pathogenesis through G3BPs.
Keywords: G3BP1; SARS-CoV-2; nucleocapsid protein; stress granule.