tetano
Editor, Senior Moderator
Life Sci Alliance
. 2022 Apr 25;5(8):e202101347.
doi: 10.26508/lsa.202101347. Print 2022 Aug.
Subgenomic RNA profiling suggests novel mechanism in coronavirus gene regulation and host adaption
Lin Lyu[SUP] 1 [/SUP], Ru Feng[SUP] 1 [/SUP], Mingnan Zhang[SUP] 1 [/SUP], Xiaoqing Xie[SUP] 1 [/SUP], Yinjing Liao[SUP] 2 [/SUP], Yanjiao Zhou[SUP] 3 [/SUP], Xiaokui Guo[SUP] 1 [/SUP], Bing Su[SUP] 1 [/SUP], Yair Dorsett[SUP] 4 [/SUP], Lei Chen[SUP] 5 [/SUP]
Affiliations
Abstract
Fundamental to viral biology is identification and annotation of viral genes and their function. Determining the level of coronavirus gene expression is inherently difficult due to the positive stranded RNA genome and the identification of subgenomic RNAs (sgRNAs) that are required for expression of most viral genes. We developed a bioinformatic pipeline to analyze metatranscriptomic data from 20 independent studies encompassing 588 individual samples and 10 coronavirus species. This comparative analysis defined a core sgRNA repertoire for SARS-CoV-2 and found novel sgRNAs that could encode functional short peptides. Relevant to coronavirus infectivity and transmission, we also observed that the ratio of Spike sgRNA to Nucleocapsid one is highest in SARS-CoV-2, among the β-coronaviruses examined. Furthermore, the adjustment of this ratio can be made by modifications to the viral RNA replication machinery, representing a form of viral gene regulation that may be involved in host adaption.
. 2022 Apr 25;5(8):e202101347.
doi: 10.26508/lsa.202101347. Print 2022 Aug.
Subgenomic RNA profiling suggests novel mechanism in coronavirus gene regulation and host adaption
Lin Lyu[SUP] 1 [/SUP], Ru Feng[SUP] 1 [/SUP], Mingnan Zhang[SUP] 1 [/SUP], Xiaoqing Xie[SUP] 1 [/SUP], Yinjing Liao[SUP] 2 [/SUP], Yanjiao Zhou[SUP] 3 [/SUP], Xiaokui Guo[SUP] 1 [/SUP], Bing Su[SUP] 1 [/SUP], Yair Dorsett[SUP] 4 [/SUP], Lei Chen[SUP] 5 [/SUP]
Affiliations
- PMID: 35470238
- DOI: 10.26508/lsa.202101347
Abstract
Fundamental to viral biology is identification and annotation of viral genes and their function. Determining the level of coronavirus gene expression is inherently difficult due to the positive stranded RNA genome and the identification of subgenomic RNAs (sgRNAs) that are required for expression of most viral genes. We developed a bioinformatic pipeline to analyze metatranscriptomic data from 20 independent studies encompassing 588 individual samples and 10 coronavirus species. This comparative analysis defined a core sgRNA repertoire for SARS-CoV-2 and found novel sgRNAs that could encode functional short peptides. Relevant to coronavirus infectivity and transmission, we also observed that the ratio of Spike sgRNA to Nucleocapsid one is highest in SARS-CoV-2, among the β-coronaviruses examined. Furthermore, the adjustment of this ratio can be made by modifications to the viral RNA replication machinery, representing a form of viral gene regulation that may be involved in host adaption.