tetano
Editor, Senior Moderator
J Gen Virol. 2019 Aug 19. doi: 10.1099/jgv.0.001314. [Epub ahead of print]
[h=1]Long-term culture of human lung adenocarcinoma A549 cells enhances the replication of human influenza A viruses.[/h] Ujie M[SUP]1[/SUP], Takada K[SUP]1[/SUP], Kiso M[SUP]1[/SUP], Sakai-Tagawa Y[SUP]1[/SUP], Ito M[SUP]1[/SUP], Nakamura K[SUP]2[/SUP], Watanabe S[SUP]2[/SUP], Imai M[SUP]1[/SUP], Kawaoka Y[SUP]3,[/SUP][SUP]4,[/SUP][SUP]1[/SUP].
[h=3]Author information[/h] 1 Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan. 2 Influenza Virus Research Center, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan. 3 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA. 4 Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
[h=3]Abstract[/h] Long-term culture of the human lung adenocarcinoma cell line A549 promotes the differentiation of these cells toward an alveolar type II cell phenotype. Here, we evaluated the susceptibility of long-term cultured A549 cells to human influenza viruses. A549 cells were cultured continuously for 25 days (D25-A549) or 1 day (D1-A549) in Ham's F12K medium. Six human influenza A viruses grew much faster in D25-A549 cells than in D1-A549 cells; however, two influenza B viruses replicated poorly in both cell types. Two avian influenza viruses replicated efficiently in both cell types, with similar titres. Expression levels of human virus receptors were higher in D25-A549 cells than in D1-A549 cells. D25-A549 cells thus more efficiently support the replication of human influenza A viruses compared with D1-A549 cells. Our data suggest that long-term cultured A549 cells will be useful for influenza A virus research.
[h=4]KEYWORDS:[/h] A549 cells; Human influenza A viruses; long-term culture; replication
PMID: 31424377 DOI: 10.1099/jgv.0.001314
[h=1]Long-term culture of human lung adenocarcinoma A549 cells enhances the replication of human influenza A viruses.[/h] Ujie M[SUP]1[/SUP], Takada K[SUP]1[/SUP], Kiso M[SUP]1[/SUP], Sakai-Tagawa Y[SUP]1[/SUP], Ito M[SUP]1[/SUP], Nakamura K[SUP]2[/SUP], Watanabe S[SUP]2[/SUP], Imai M[SUP]1[/SUP], Kawaoka Y[SUP]3,[/SUP][SUP]4,[/SUP][SUP]1[/SUP].
[h=3]Author information[/h] 1 Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan. 2 Influenza Virus Research Center, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan. 3 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA. 4 Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
[h=3]Abstract[/h] Long-term culture of the human lung adenocarcinoma cell line A549 promotes the differentiation of these cells toward an alveolar type II cell phenotype. Here, we evaluated the susceptibility of long-term cultured A549 cells to human influenza viruses. A549 cells were cultured continuously for 25 days (D25-A549) or 1 day (D1-A549) in Ham's F12K medium. Six human influenza A viruses grew much faster in D25-A549 cells than in D1-A549 cells; however, two influenza B viruses replicated poorly in both cell types. Two avian influenza viruses replicated efficiently in both cell types, with similar titres. Expression levels of human virus receptors were higher in D25-A549 cells than in D1-A549 cells. D25-A549 cells thus more efficiently support the replication of human influenza A viruses compared with D1-A549 cells. Our data suggest that long-term cultured A549 cells will be useful for influenza A virus research.
[h=4]KEYWORDS:[/h] A549 cells; Human influenza A viruses; long-term culture; replication
PMID: 31424377 DOI: 10.1099/jgv.0.001314