tetano
Editor, Senior Moderator
Luminescence
. 2024 Jan;39(1):e4675.
doi: 10.1002/bio.4675. Amplified detection of SARS-COV-2 B.1.1.529 (Omicron) gene oligonucleotides based on exonuclease III-aided MoS[SUB]2[/SUB] /AIE nanoprobes
Gerile Oudeng[SUP] 1 [/SUP], Junguo Ni[SUP] 2 [/SUP], Hao Wu[SUP] 3 [/SUP], Honglian Wu[SUP] 2 [/SUP], Mo Yang[SUP] 2 [/SUP], Chunyi Wen[SUP] 2 [/SUP], Yuanwei Wang[SUP] 1 [/SUP], Hui Tan[SUP] 1 [/SUP]
Affiliations
The coronavirus disease-2019 pandemic reflects the underdevelopment of point-of-care diagnostic technology. Nuclei acid (NA) detection is the "gold standard" method for the early diagnosis of the B.1.1.529 (Omicron) variant of severe acute respiratory syndrome-coronavirus disease-2. Polymerase chain reaction is the main method for NA detection but requires considerable manpower and sample processing taking ≥ 3 h. To simplify the operation processes and reduce the detection time, exonuclease III (Exo III)-aided MoS[SUB]2[/SUB] /AIE nanoprobes were developed for rapid and sensitive detection of the oligonucleotides of Omicron. Molybdenum disulfide (MoS[SUB]2[/SUB] ) nanosheets with excellent optical absorbance and distinguishable affinity to single-strand and duplex DNAs were applied as quenchers, and aggregation-induced emission (AIE) molecules with high luminous efficiency were designed as donor in fluorescence resonance energy transfer-based nanoprobes. Exo III with catalytic capability was used for signal amplification to increase the sensitivity of detection. The composite nanoprobes detected the mutated nucleocapsid (N)-gene and spike (S)-gene oligonucleotides of Omicron within 40 min with a limit of detection of 4.7 pM, and showed great potential for application in community medicine.
Keywords: Omicron gene detection; aggregation-induced emission; amplified gene detection; fluorescence resonance energy transfer; molybdenum disulfide nanosheets.
. 2024 Jan;39(1):e4675.
doi: 10.1002/bio.4675. Amplified detection of SARS-COV-2 B.1.1.529 (Omicron) gene oligonucleotides based on exonuclease III-aided MoS[SUB]2[/SUB] /AIE nanoprobes
Gerile Oudeng[SUP] 1 [/SUP], Junguo Ni[SUP] 2 [/SUP], Hao Wu[SUP] 3 [/SUP], Honglian Wu[SUP] 2 [/SUP], Mo Yang[SUP] 2 [/SUP], Chunyi Wen[SUP] 2 [/SUP], Yuanwei Wang[SUP] 1 [/SUP], Hui Tan[SUP] 1 [/SUP]
Affiliations
- PMID: 38286603
- DOI: 10.1002/bio.4675
The coronavirus disease-2019 pandemic reflects the underdevelopment of point-of-care diagnostic technology. Nuclei acid (NA) detection is the "gold standard" method for the early diagnosis of the B.1.1.529 (Omicron) variant of severe acute respiratory syndrome-coronavirus disease-2. Polymerase chain reaction is the main method for NA detection but requires considerable manpower and sample processing taking ≥ 3 h. To simplify the operation processes and reduce the detection time, exonuclease III (Exo III)-aided MoS[SUB]2[/SUB] /AIE nanoprobes were developed for rapid and sensitive detection of the oligonucleotides of Omicron. Molybdenum disulfide (MoS[SUB]2[/SUB] ) nanosheets with excellent optical absorbance and distinguishable affinity to single-strand and duplex DNAs were applied as quenchers, and aggregation-induced emission (AIE) molecules with high luminous efficiency were designed as donor in fluorescence resonance energy transfer-based nanoprobes. Exo III with catalytic capability was used for signal amplification to increase the sensitivity of detection. The composite nanoprobes detected the mutated nucleocapsid (N)-gene and spike (S)-gene oligonucleotides of Omicron within 40 min with a limit of detection of 4.7 pM, and showed great potential for application in community medicine.
Keywords: Omicron gene detection; aggregation-induced emission; amplified gene detection; fluorescence resonance energy transfer; molybdenum disulfide nanosheets.