tetano
Editor, Senior Moderator
Am J Physiol Lung Cell Mol Physiol. 1997 Dec 1;273(6):L1156-L1166. doi: 10.1152/ajplung.1997.273.6.L1156.
[h=1]Mechanisms of anti-influenza activity of surfactant proteins A and D: comparison with serum collectins.[/h] Hartshorn KL[SUP]1[/SUP], White MR[SUP]1[/SUP], Shepherd V[SUP]2[/SUP], Reid K[SUP]3[/SUP], Jensenius JC[SUP]4[/SUP], Crouch EC[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The present study provides the first direct comparison of anti-influenza A virus (IAV) activities of the collectins surfactant protein (SP) A and SP-D, mannose-binding lectin (MBL), and conglutinin. SP-D, MBL, and conglutinin inhibited IAV hemagglutination activity with a greater potency than and by a distinct mechanism from SP-A. Although isolated trimeric SP-D carbohydrate recognition domains inhibited hemagglutination activity, preparations of SP-D also containing the collagen domain and NH[SUB]2[/SUB] terminus caused greater inhibition. In contrast to SP-A (or nonmultimerized SP-D), absence of the N-linked attachment did not effect interactions of multimerized SP-D with IAV. SP-D, SP-A, and conglutinin caused viral precipitation through formation of massive viral aggregates, whereas MBL formed aggregates of smaller size that did not precipitate. All of the collectins enhanced IAV binding to neutrophils; however, in the case of MBL, this effect was modest compared with the binding enhancement induced by SP-D or conglutinin. These studies clarify the structural requirements for viral inhibition by SP-D and reveal significant differences in the mechanisms of anti-IAV activity among the collectins.
[h=4]KEYWORDS:[/h] influenza A virus; mannose-binding lectin; neutrophils
PMID: 29585337 DOI: 10.1152/ajplung.1997.273.6.L1156
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[h=1]Mechanisms of anti-influenza activity of surfactant proteins A and D: comparison with serum collectins.[/h] Hartshorn KL[SUP]1[/SUP], White MR[SUP]1[/SUP], Shepherd V[SUP]2[/SUP], Reid K[SUP]3[/SUP], Jensenius JC[SUP]4[/SUP], Crouch EC[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The present study provides the first direct comparison of anti-influenza A virus (IAV) activities of the collectins surfactant protein (SP) A and SP-D, mannose-binding lectin (MBL), and conglutinin. SP-D, MBL, and conglutinin inhibited IAV hemagglutination activity with a greater potency than and by a distinct mechanism from SP-A. Although isolated trimeric SP-D carbohydrate recognition domains inhibited hemagglutination activity, preparations of SP-D also containing the collagen domain and NH[SUB]2[/SUB] terminus caused greater inhibition. In contrast to SP-A (or nonmultimerized SP-D), absence of the N-linked attachment did not effect interactions of multimerized SP-D with IAV. SP-D, SP-A, and conglutinin caused viral precipitation through formation of massive viral aggregates, whereas MBL formed aggregates of smaller size that did not precipitate. All of the collectins enhanced IAV binding to neutrophils; however, in the case of MBL, this effect was modest compared with the binding enhancement induced by SP-D or conglutinin. These studies clarify the structural requirements for viral inhibition by SP-D and reveal significant differences in the mechanisms of anti-IAV activity among the collectins.
[h=4]KEYWORDS:[/h] influenza A virus; mannose-binding lectin; neutrophils
PMID: 29585337 DOI: 10.1152/ajplung.1997.273.6.L1156
Free full text