tetano
Editor, Senior Moderator
Microbiol Spectr
. 2023 Nov 27:e0246923.
doi: 10.1128/spectrum.02469-23. Online ahead of print. Spatiotemporal analysis of SARS-CoV-2 infection reveals an expansive wave of monocyte-derived macrophages associated with vascular damage and virus clearance in hamster lungs
Ola Bagato[SUP] 1 2 [/SUP], Anne Balkema-Buschmann[SUP] 3 [/SUP], Daniel Todt[SUP] 4 [/SUP], Saskia Weber[SUP] 5 [/SUP], André Gömer[SUP] 4 [/SUP], Bingqian Qu[SUP] 6 [/SUP], Csaba Miskey[SUP] 7 [/SUP], Zoltan Ivics[SUP] 7 [/SUP], Thomas C Mettenleiter[SUP] 8 [/SUP], Stefan Finke[SUP] 1 [/SUP], Richard J P Brown[SUP] 4 6 [/SUP], Angele Breithaupt[SUP] 9 [/SUP], Dmitry S Ushakov[SUP] 1 [/SUP]
Affiliations
We present the first study of the 3D kinetics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and the early host response in a large lung volume using a combination of tissue imaging and transcriptomics. This approach allowed us to make a number of important findings:Spatially restricted antiviral response is shown, including the formation of monocytic macrophage clusters and upregulation of the major histocompatibility complex II in infected epithelial cells.The monocyte-derived macrophages are linked to SARS-CoV-2 clearance, and the appearance of these cells is associated with post-infection endothelial damage; thus, we shed light on the role of these cells in infected tissue.An early onset of tissue repair occurring simultaneously with inflammatory and necrotizing processes provides the basis for longer-term alterations in the lungs.
Keywords: COVID-19; Golden Syrian hamster; SARS-CoV-2; innate immunity; light sheet fluorescence microscopy; lung immunity; macrophages; transcriptomics.
. 2023 Nov 27:e0246923.
doi: 10.1128/spectrum.02469-23. Online ahead of print. Spatiotemporal analysis of SARS-CoV-2 infection reveals an expansive wave of monocyte-derived macrophages associated with vascular damage and virus clearance in hamster lungs
Ola Bagato[SUP] 1 2 [/SUP], Anne Balkema-Buschmann[SUP] 3 [/SUP], Daniel Todt[SUP] 4 [/SUP], Saskia Weber[SUP] 5 [/SUP], André Gömer[SUP] 4 [/SUP], Bingqian Qu[SUP] 6 [/SUP], Csaba Miskey[SUP] 7 [/SUP], Zoltan Ivics[SUP] 7 [/SUP], Thomas C Mettenleiter[SUP] 8 [/SUP], Stefan Finke[SUP] 1 [/SUP], Richard J P Brown[SUP] 4 6 [/SUP], Angele Breithaupt[SUP] 9 [/SUP], Dmitry S Ushakov[SUP] 1 [/SUP]
Affiliations
- PMID: 38009950
- DOI: 10.1128/spectrum.02469-23
We present the first study of the 3D kinetics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and the early host response in a large lung volume using a combination of tissue imaging and transcriptomics. This approach allowed us to make a number of important findings:Spatially restricted antiviral response is shown, including the formation of monocytic macrophage clusters and upregulation of the major histocompatibility complex II in infected epithelial cells.The monocyte-derived macrophages are linked to SARS-CoV-2 clearance, and the appearance of these cells is associated with post-infection endothelial damage; thus, we shed light on the role of these cells in infected tissue.An early onset of tissue repair occurring simultaneously with inflammatory and necrotizing processes provides the basis for longer-term alterations in the lungs.
Keywords: COVID-19; Golden Syrian hamster; SARS-CoV-2; innate immunity; light sheet fluorescence microscopy; lung immunity; macrophages; transcriptomics.