tetano
Editor, Senior Moderator
Mol Biol Rep
. 2022 Oct 29.
doi: 10.1007/s11033-022-07981-2. Online ahead of print.
Retinoic acid-inducible gene 1 (RIG-1) and IFN-β promoter stimulator-1 (IPS-1) significantly down-regulated in the severe coronavirus disease 2019 (COVID-19)
Peyman Nahavandi-Parizi[SUP] 1 [/SUP], Ashraf Kariminik[SUP] 2 [/SUP], Maryam Montazeri[SUP] 1 [/SUP]
Affiliations
Abstract
Introduction: Retinoic acid-inducible gene 1 (RIG-1) and melanoma differentiation-associated protein 5 (MDA5) are the well-known cytoplasmic sensors that recognize microbial DNA or RNA and active down-stream molecules, including IFN-β promoter stimulator-1 (IPS-1) and receptor interacting protein 1 (RIP1). The roles played by the networked molecules on the infection with SARS-CoV-2 needs more investigations.
Material and method: In this project MDA5, RIG-1, IPS-1 and RIP1 mRNA levels were evaluated in 45 hospitalized patients suffering from coronavirus disease of 2019 (COVID-19) and 45 healthy subjects using Real Time-qPCR technique.
Result: The results showed significant decreased RIG-1 and IPS-1 in the SARS-CoV-2 infected patients when compared to healthy cases. MDA5 and RIP1 did not change when compared two groups. Male patients had similar expression of MDA5, RIG-1, IPS-1 and RIP1 when compared to female patients.
Conclusion: Based on the results, it seems that RIG-1 and its signaling molecule, IPS-1, play key roles in the peripheral blood immune cells against SARS-CoV-2 and, their down-regulation may be induced by the virus to escape from immune responses.
Keywords: IPS-1; Innate immunity; MDA5; RIG-1; RIP1; SARS-CoV-2.
. 2022 Oct 29.
doi: 10.1007/s11033-022-07981-2. Online ahead of print.
Retinoic acid-inducible gene 1 (RIG-1) and IFN-β promoter stimulator-1 (IPS-1) significantly down-regulated in the severe coronavirus disease 2019 (COVID-19)
Peyman Nahavandi-Parizi[SUP] 1 [/SUP], Ashraf Kariminik[SUP] 2 [/SUP], Maryam Montazeri[SUP] 1 [/SUP]
Affiliations
- PMID: 36309611
- DOI: 10.1007/s11033-022-07981-2
Abstract
Introduction: Retinoic acid-inducible gene 1 (RIG-1) and melanoma differentiation-associated protein 5 (MDA5) are the well-known cytoplasmic sensors that recognize microbial DNA or RNA and active down-stream molecules, including IFN-β promoter stimulator-1 (IPS-1) and receptor interacting protein 1 (RIP1). The roles played by the networked molecules on the infection with SARS-CoV-2 needs more investigations.
Material and method: In this project MDA5, RIG-1, IPS-1 and RIP1 mRNA levels were evaluated in 45 hospitalized patients suffering from coronavirus disease of 2019 (COVID-19) and 45 healthy subjects using Real Time-qPCR technique.
Result: The results showed significant decreased RIG-1 and IPS-1 in the SARS-CoV-2 infected patients when compared to healthy cases. MDA5 and RIP1 did not change when compared two groups. Male patients had similar expression of MDA5, RIG-1, IPS-1 and RIP1 when compared to female patients.
Conclusion: Based on the results, it seems that RIG-1 and its signaling molecule, IPS-1, play key roles in the peripheral blood immune cells against SARS-CoV-2 and, their down-regulation may be induced by the virus to escape from immune responses.
Keywords: IPS-1; Innate immunity; MDA5; RIG-1; RIP1; SARS-CoV-2.