tetano
Editor, Senior Moderator
Mol Immunol
. 2023 Aug 28;162:54-63.
doi: 10.1016/j.molimm.2023.08.005. Online ahead of print. Altered T-cell receptor β repertoire in adults with SARS CoV-2 inactivated vaccine of BBIBP-CorV
Zhihui Quan[SUP] 1 [/SUP], Aihong Qi[SUP] 2 [/SUP], Shuwen Ma[SUP] 2 [/SUP], Yanling Li[SUP] 3 [/SUP], Hui Chen[SUP] 3 [/SUP], Xue Yu[SUP] 1 [/SUP], Tingyan Dong[SUP] 4 [/SUP], Kui Li[SUP] 5 [/SUP], Yurong Qiu[SUP] 6 [/SUP]
Affiliations
Objective: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the prolonged and widespread epidemic of coronavirus disease 2019 (COVID-19). The inactivated BBIBP-CorV vaccine has shown safety, efficacy and immunogenicity against COVID-19 in in-vitro studies and clinical trials. However, the characteristics changes of the TCRβ repertoire in patients receiving BBIBP-CorV remain unclear.
Methods: TCRβ repertoire difference were analyzed between 54 uninfected subjects who received a third dose of the enhanced BBIBP-CorV vaccine and the 16 healthy donors who did not receive the vaccine and 44 COVID-19 patients with different courses of disease (asymptomatic, symptomatic and convalescent). Furthermore, antibody response, anti-inflammatory and pro-inflammatory cytokines also were examined.
Results: We found that the third dose inactivated coronavirus vaccine induced widespread changes including the increased TCRβ repertoire diversity, a much shorter CDR3 length and high usage of V-J genes segments. Meanwhile, the vaccine-responding clones were also predicted. The results of the antibody response showed that 90.7 % of the vaccinated individuals were positive for NAb seroconversion and 88.9 % for IgG antibody about 60 days after the third dose. The concentration of IL-2 increased significantly compared to baseline inoculation.
Conclusion: Altered TCRβ repertoire in adults with SARS CoV-2 inactivated vaccine of BBIBP-CorV clarified the specific immunity induced by inactivated vaccines. Our research provides insights into the adaptive immune response induced by the new inactivated SARS-CoV-2 vaccine and strengthens the development of immunotherapy.
Keywords: Antibody response; COVID-19; Inflammatory cytokines; SARS-CoV-2 vaccine; TCRβ repertoire.
. 2023 Aug 28;162:54-63.
doi: 10.1016/j.molimm.2023.08.005. Online ahead of print. Altered T-cell receptor β repertoire in adults with SARS CoV-2 inactivated vaccine of BBIBP-CorV
Zhihui Quan[SUP] 1 [/SUP], Aihong Qi[SUP] 2 [/SUP], Shuwen Ma[SUP] 2 [/SUP], Yanling Li[SUP] 3 [/SUP], Hui Chen[SUP] 3 [/SUP], Xue Yu[SUP] 1 [/SUP], Tingyan Dong[SUP] 4 [/SUP], Kui Li[SUP] 5 [/SUP], Yurong Qiu[SUP] 6 [/SUP]
Affiliations
- PMID: 37647774
- DOI: 10.1016/j.molimm.2023.08.005
Objective: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the prolonged and widespread epidemic of coronavirus disease 2019 (COVID-19). The inactivated BBIBP-CorV vaccine has shown safety, efficacy and immunogenicity against COVID-19 in in-vitro studies and clinical trials. However, the characteristics changes of the TCRβ repertoire in patients receiving BBIBP-CorV remain unclear.
Methods: TCRβ repertoire difference were analyzed between 54 uninfected subjects who received a third dose of the enhanced BBIBP-CorV vaccine and the 16 healthy donors who did not receive the vaccine and 44 COVID-19 patients with different courses of disease (asymptomatic, symptomatic and convalescent). Furthermore, antibody response, anti-inflammatory and pro-inflammatory cytokines also were examined.
Results: We found that the third dose inactivated coronavirus vaccine induced widespread changes including the increased TCRβ repertoire diversity, a much shorter CDR3 length and high usage of V-J genes segments. Meanwhile, the vaccine-responding clones were also predicted. The results of the antibody response showed that 90.7 % of the vaccinated individuals were positive for NAb seroconversion and 88.9 % for IgG antibody about 60 days after the third dose. The concentration of IL-2 increased significantly compared to baseline inoculation.
Conclusion: Altered TCRβ repertoire in adults with SARS CoV-2 inactivated vaccine of BBIBP-CorV clarified the specific immunity induced by inactivated vaccines. Our research provides insights into the adaptive immune response induced by the new inactivated SARS-CoV-2 vaccine and strengthens the development of immunotherapy.
Keywords: Antibody response; COVID-19; Inflammatory cytokines; SARS-CoV-2 vaccine; TCRβ repertoire.