tetano
Editor, Senior Moderator
Commun Biol. 2020 May 8;3(1):223. doi: 10.1038/s42003-020-0955-8.
Monocyte apoptotic bodies are vehicles for influenza A virus propagation.
Atkin-Smith GK[SUP]1[/SUP], Duan M[SUP]2[/SUP], Zanker DJ[SUP]1[/SUP], Loh L[SUP]3[/SUP], Nguyen THO[SUP]3[/SUP], Koutsakos M[SUP]3[/SUP], Nguyen T[SUP]1[/SUP], Jiang X[SUP]1[/SUP], Carrera J[SUP]3[/SUP], Phan TK[SUP]1[/SUP], Liu C[SUP]1[/SUP], Paone S[SUP]1[/SUP], Oveissi S[SUP]1[/SUP], Hodge AL[SUP]1[/SUP], Baxter AA[SUP]1[/SUP], Kedzierska K[SUP]3[/SUP], Mackenzie JM[SUP]3[/SUP], Hulett MD[SUP]1[/SUP], Bilsel P[SUP]4[/SUP], Chen W[SUP]5[/SUP], Poon IKH[SUP]6[/SUP].
Author information
Abstract
The disassembly of apoptotic cells into small membrane-bound vesicles termed apoptotic bodies (ApoBDs) is a hallmark of apoptosis; however, the functional significance of this process is not well defined. We recently discovered a new membrane protrusion (termed beaded apoptopodia) generated by apoptotic monocytes which fragments to release an abundance of ApoBDs. To investigate the function of apoptotic monocyte disassembly, we used influenza A virus (IAV) infection as a proof-of-concept model, as IAV commonly infects monocytes in physiological settings. We show that ApoBDs generated from IAV-infected monocytes contained IAV mRNA, protein and virions and consequently, could facilitate viral propagation in vitro and in vivo, and induce a robust antiviral immune response. We also identified an antipsychotic, Haloperidol, as an unexpected inhibitor of monocyte cell disassembly which could impair ApoBD-mediated viral propagation under in vitro conditions. Together, this study reveals a previously unrecognised function of apoptotic monocyte disassembly in the pathogenesis of IAV infections.
PMID:32385344DOI:10.1038/s42003-020-0955-8
Monocyte apoptotic bodies are vehicles for influenza A virus propagation.
Atkin-Smith GK[SUP]1[/SUP], Duan M[SUP]2[/SUP], Zanker DJ[SUP]1[/SUP], Loh L[SUP]3[/SUP], Nguyen THO[SUP]3[/SUP], Koutsakos M[SUP]3[/SUP], Nguyen T[SUP]1[/SUP], Jiang X[SUP]1[/SUP], Carrera J[SUP]3[/SUP], Phan TK[SUP]1[/SUP], Liu C[SUP]1[/SUP], Paone S[SUP]1[/SUP], Oveissi S[SUP]1[/SUP], Hodge AL[SUP]1[/SUP], Baxter AA[SUP]1[/SUP], Kedzierska K[SUP]3[/SUP], Mackenzie JM[SUP]3[/SUP], Hulett MD[SUP]1[/SUP], Bilsel P[SUP]4[/SUP], Chen W[SUP]5[/SUP], Poon IKH[SUP]6[/SUP].
Author information
Abstract
The disassembly of apoptotic cells into small membrane-bound vesicles termed apoptotic bodies (ApoBDs) is a hallmark of apoptosis; however, the functional significance of this process is not well defined. We recently discovered a new membrane protrusion (termed beaded apoptopodia) generated by apoptotic monocytes which fragments to release an abundance of ApoBDs. To investigate the function of apoptotic monocyte disassembly, we used influenza A virus (IAV) infection as a proof-of-concept model, as IAV commonly infects monocytes in physiological settings. We show that ApoBDs generated from IAV-infected monocytes contained IAV mRNA, protein and virions and consequently, could facilitate viral propagation in vitro and in vivo, and induce a robust antiviral immune response. We also identified an antipsychotic, Haloperidol, as an unexpected inhibitor of monocyte cell disassembly which could impair ApoBD-mediated viral propagation under in vitro conditions. Together, this study reveals a previously unrecognised function of apoptotic monocyte disassembly in the pathogenesis of IAV infections.
PMID:32385344DOI:10.1038/s42003-020-0955-8