tetano
Editor, Senior Moderator
Virus Res. 2018 May 3. pii: S0168-1702(17)30905-X. doi: 10.1016/j.virusres.2018.05.001. [Epub ahead of print]
[h=1]N-Myc Downstream-Regulated Gene 1 Facilitates Influenza A Virus Replication by Suppressing Canonical NF-κB Signaling.[/h] Chen L[SUP]1[/SUP], Xing C[SUP]2[/SUP], Ma G[SUP]1[/SUP], Luo J[SUP]3[/SUP], Su W[SUP]1[/SUP], Li M[SUP]1[/SUP], Shi Q[SUP]4[/SUP], He H[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The highly pathogenic avian influenza (HPAI) A/H5N1 virus hijacks host cellular machinery to complete its life cycle; identification of the host factors involved in viral replication may facilitate antiviral drug development. Here, we first characterize a metastasis suppressor, N-myc downstream-regulated gene 1 (NDRG1), and showed that it plays a crucial role in H5N1 viral replication. We found that H5N1 infection upregulated NDRG1 mRNA and protein expression. Overexpression of NDRG1 released approximately 4-fold more virions compared to the control group, whereas knockdown of NDRG1 resulted in a drop in viral RNA and protein production. Further investigation revealed that NDRG1 facilitated HPAI A/H5N1 viral replication by suppressing the canonical NF-κB signaling pathway. Furthermore, our results also showed that the NDRG1 mRNA level was mainly stimulated by M1 and PB1 viral proteins. Overall, our results suggest that NDRG1 plays a positive role in HPAI replication by suppressing the canonical NF-κB signaling pathway.
[h=4]KEYWORDS:[/h] HPAI A/H5N1 virus; Innate immunity response; NDRG1; NF-κB
PMID: 29730307 DOI: 10.1016/j.virusres.2018.05.001
[h=1]N-Myc Downstream-Regulated Gene 1 Facilitates Influenza A Virus Replication by Suppressing Canonical NF-κB Signaling.[/h] Chen L[SUP]1[/SUP], Xing C[SUP]2[/SUP], Ma G[SUP]1[/SUP], Luo J[SUP]3[/SUP], Su W[SUP]1[/SUP], Li M[SUP]1[/SUP], Shi Q[SUP]4[/SUP], He H[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The highly pathogenic avian influenza (HPAI) A/H5N1 virus hijacks host cellular machinery to complete its life cycle; identification of the host factors involved in viral replication may facilitate antiviral drug development. Here, we first characterize a metastasis suppressor, N-myc downstream-regulated gene 1 (NDRG1), and showed that it plays a crucial role in H5N1 viral replication. We found that H5N1 infection upregulated NDRG1 mRNA and protein expression. Overexpression of NDRG1 released approximately 4-fold more virions compared to the control group, whereas knockdown of NDRG1 resulted in a drop in viral RNA and protein production. Further investigation revealed that NDRG1 facilitated HPAI A/H5N1 viral replication by suppressing the canonical NF-κB signaling pathway. Furthermore, our results also showed that the NDRG1 mRNA level was mainly stimulated by M1 and PB1 viral proteins. Overall, our results suggest that NDRG1 plays a positive role in HPAI replication by suppressing the canonical NF-κB signaling pathway.
[h=4]KEYWORDS:[/h] HPAI A/H5N1 virus; Innate immunity response; NDRG1; NF-κB
PMID: 29730307 DOI: 10.1016/j.virusres.2018.05.001