tetano
Editor, Senior Moderator
Nat Commun
. 2021 May 19;12(1):2938.
doi: 10.1038/s41467-021-23074-3.
Cross-reactive serum and memory B-cell responses to spike protein in SARS-CoV-2 and endemic coronavirus infection
Ge Song[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Wan-Ting He[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Sean Callaghan[SUP] 1 2 3 [/SUP], Fabio Anzanello[SUP] 1 2 3 [/SUP], Deli Huang[SUP] 1 [/SUP], James Ricketts[SUP] 1 [/SUP], Jonathan L Torres[SUP] 4 [/SUP], Nathan Beutler[SUP] 1 [/SUP], Linghang Peng[SUP] 1 [/SUP], Sirena Vargas[SUP] 1 2 3 [/SUP], Jon Cassell[SUP] 1 2 3 [/SUP], Mara Parren[SUP] 1 [/SUP], Linlin Yang[SUP] 1 [/SUP], Caroline Ignacio[SUP] 5 [/SUP], Davey M Smith[SUP] 5 [/SUP], James E Voss[SUP] 1 [/SUP], David Nemazee[SUP] 1 [/SUP], Andrew B Ward[SUP] 2 3 4 [/SUP], Thomas Rogers[SUP] 1 5 [/SUP], Dennis R Burton[SUP] 6 7 8 9 [/SUP], Raiees Andrabi[SUP] 10 11 12 [/SUP]
Affiliations
Abstract
Pre-existing immunity to seasonal endemic coronaviruses could have profound consequences for antibody responses to SARS-CoV-2, induced from natural infection or vaccination. A first step to establish whether pre-existing responses can impact SARS-CoV-2 infection is to understand the nature and extent of cross-reactivity in humans to coronaviruses. Here we compare serum antibody and memory B cell responses to coronavirus spike proteins from pre-pandemic and SARS-CoV-2 convalescent donors using binding and functional assays. We show weak evidence of pre-existing SARS-CoV-2 cross-reactive serum antibodies in pre-pandemic donors. However, we find evidence of pre-existing cross-reactive memory B cells that are activated during SARS-CoV-2 infection. Monoclonal antibodies show varying degrees of cross-reactivity with betacoronaviruses, including SARS-CoV-1 and endemic coronaviruses. We identify one cross-reactive neutralizing antibody specific to the S2 subunit of the S protein. Our results suggest that pre-existing immunity to endemic coronaviruses should be considered in evaluating antibody responses to SARS-CoV-2.
. 2021 May 19;12(1):2938.
doi: 10.1038/s41467-021-23074-3.
Cross-reactive serum and memory B-cell responses to spike protein in SARS-CoV-2 and endemic coronavirus infection
Ge Song[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Wan-Ting He[SUP] #[/SUP][SUP] 1 2 3 [/SUP], Sean Callaghan[SUP] 1 2 3 [/SUP], Fabio Anzanello[SUP] 1 2 3 [/SUP], Deli Huang[SUP] 1 [/SUP], James Ricketts[SUP] 1 [/SUP], Jonathan L Torres[SUP] 4 [/SUP], Nathan Beutler[SUP] 1 [/SUP], Linghang Peng[SUP] 1 [/SUP], Sirena Vargas[SUP] 1 2 3 [/SUP], Jon Cassell[SUP] 1 2 3 [/SUP], Mara Parren[SUP] 1 [/SUP], Linlin Yang[SUP] 1 [/SUP], Caroline Ignacio[SUP] 5 [/SUP], Davey M Smith[SUP] 5 [/SUP], James E Voss[SUP] 1 [/SUP], David Nemazee[SUP] 1 [/SUP], Andrew B Ward[SUP] 2 3 4 [/SUP], Thomas Rogers[SUP] 1 5 [/SUP], Dennis R Burton[SUP] 6 7 8 9 [/SUP], Raiees Andrabi[SUP] 10 11 12 [/SUP]
Affiliations
- PMID: 34011939
- DOI: 10.1038/s41467-021-23074-3
Abstract
Pre-existing immunity to seasonal endemic coronaviruses could have profound consequences for antibody responses to SARS-CoV-2, induced from natural infection or vaccination. A first step to establish whether pre-existing responses can impact SARS-CoV-2 infection is to understand the nature and extent of cross-reactivity in humans to coronaviruses. Here we compare serum antibody and memory B cell responses to coronavirus spike proteins from pre-pandemic and SARS-CoV-2 convalescent donors using binding and functional assays. We show weak evidence of pre-existing SARS-CoV-2 cross-reactive serum antibodies in pre-pandemic donors. However, we find evidence of pre-existing cross-reactive memory B cells that are activated during SARS-CoV-2 infection. Monoclonal antibodies show varying degrees of cross-reactivity with betacoronaviruses, including SARS-CoV-1 and endemic coronaviruses. We identify one cross-reactive neutralizing antibody specific to the S2 subunit of the S protein. Our results suggest that pre-existing immunity to endemic coronaviruses should be considered in evaluating antibody responses to SARS-CoV-2.