tetano
Editor, Senior Moderator
Nat Immunol
. 2020 Sep 30.
doi: 10.1038/s41590-020-00808-x. Online ahead of print.
SARS-CoV-2-derived peptides define heterologous and COVID-19-induced T cell recognition
Annika Nelde[SUP] 1 2 3 [/SUP], Tatjana Bilich[SUP] 1 2 3 [/SUP], Jonas S Heitmann[SUP] 1 3 [/SUP], Yacine Maringer[SUP] 1 2 3 [/SUP], Helmut R Salih[SUP] 1 3 4 [/SUP], Malte Roerden[SUP] 2 3 5 [/SUP], Maren L?bke[SUP] 2 [/SUP], Jens Bauer[SUP] 1 2 [/SUP], Jonas Rieth[SUP] 1 2 [/SUP], Marcel Wacker[SUP] 1 2 [/SUP], Andreas Peter[SUP] 6 [/SUP], Sebastian H?rber[SUP] 6 [/SUP], Bjoern Traenkle[SUP] 7 [/SUP], Philipp D Kaiser[SUP] 7 [/SUP], Ulrich Rothbauer[SUP] 7 8 [/SUP], Matthias Becker[SUP] 7 [/SUP], Daniel Junker[SUP] 7 [/SUP], G?rard Krause[SUP] 9 10 11 [/SUP], Monika Strengert[SUP] 9 10 [/SUP], Nicole Schneiderhan-Marra[SUP] 7 [/SUP], Markus F Templin[SUP] 7 [/SUP], Thomas O Joos[SUP] 7 [/SUP], Daniel J Kowalewski[SUP] 12 [/SUP], Vlatka Stos-Zweifel[SUP] 12 [/SUP], Michael Fehr[SUP] 2 [/SUP], Armin Rabsteyn[SUP] 3 13 [/SUP], Valbona Mirakaj[SUP] 14 [/SUP], Julia Karbach[SUP] 15 [/SUP], Elke J?ger[SUP] 15 [/SUP], Michael Graf[SUP] 16 [/SUP], Lena-Christin Gruber[SUP] 1 [/SUP], David Rachfalski[SUP] 1 [/SUP], Beate Preu?[SUP] 5 [/SUP], Ilona Hagelstein[SUP] 1 3 [/SUP], Melanie M?rklin[SUP] 1 3 [/SUP], Tamam Bakchoul[SUP] 17 [/SUP], C?cile Gouttefangeas[SUP] 2 3 4 [/SUP], Oliver Kohlbacher[SUP] 16 18 19 20 [/SUP], Reinhild Klein[SUP] 5 [/SUP], Stefan Stevanović[SUP] 2 4 [/SUP], Hans-Georg Rammensee[SUP] 2 3 4 [/SUP], Juliane S Walz[SUP] 21 22 23 [/SUP]
Affiliations
Abstract
T cell immunity is central for the control of viral infections. To characterize T cell immunity, but also for the development of vaccines, identification of exact viral T cell epitopes is fundamental. Here we identify and characterize multiple dominant and subdominant SARS-CoV-2 HLA class I and HLA-DR peptides as potential T cell epitopes in COVID-19 convalescent and unexposed individuals. SARS-CoV-2-specific peptides enabled detection of post-infectious T cell immunity, even in seronegative convalescent individuals. Cross-reactive SARS-CoV-2 peptides revealed pre-existing T cell responses in 81% of unexposed individuals and validated similarity with common cold coronaviruses, providing a functional basis for heterologous immunity in SARS-CoV-2 infection. Diversity of SARS-CoV-2 T cell responses was associated with mild symptoms of COVID-19, providing evidence that immunity requires recognition of multiple epitopes. Together, the proposed SARS-CoV-2 T cell epitopes enable identification of heterologous and post-infectious T cell immunity and facilitate development of diagnostic, preventive and therapeutic measures for COVID-19.
. 2020 Sep 30.
doi: 10.1038/s41590-020-00808-x. Online ahead of print.
SARS-CoV-2-derived peptides define heterologous and COVID-19-induced T cell recognition
Annika Nelde[SUP] 1 2 3 [/SUP], Tatjana Bilich[SUP] 1 2 3 [/SUP], Jonas S Heitmann[SUP] 1 3 [/SUP], Yacine Maringer[SUP] 1 2 3 [/SUP], Helmut R Salih[SUP] 1 3 4 [/SUP], Malte Roerden[SUP] 2 3 5 [/SUP], Maren L?bke[SUP] 2 [/SUP], Jens Bauer[SUP] 1 2 [/SUP], Jonas Rieth[SUP] 1 2 [/SUP], Marcel Wacker[SUP] 1 2 [/SUP], Andreas Peter[SUP] 6 [/SUP], Sebastian H?rber[SUP] 6 [/SUP], Bjoern Traenkle[SUP] 7 [/SUP], Philipp D Kaiser[SUP] 7 [/SUP], Ulrich Rothbauer[SUP] 7 8 [/SUP], Matthias Becker[SUP] 7 [/SUP], Daniel Junker[SUP] 7 [/SUP], G?rard Krause[SUP] 9 10 11 [/SUP], Monika Strengert[SUP] 9 10 [/SUP], Nicole Schneiderhan-Marra[SUP] 7 [/SUP], Markus F Templin[SUP] 7 [/SUP], Thomas O Joos[SUP] 7 [/SUP], Daniel J Kowalewski[SUP] 12 [/SUP], Vlatka Stos-Zweifel[SUP] 12 [/SUP], Michael Fehr[SUP] 2 [/SUP], Armin Rabsteyn[SUP] 3 13 [/SUP], Valbona Mirakaj[SUP] 14 [/SUP], Julia Karbach[SUP] 15 [/SUP], Elke J?ger[SUP] 15 [/SUP], Michael Graf[SUP] 16 [/SUP], Lena-Christin Gruber[SUP] 1 [/SUP], David Rachfalski[SUP] 1 [/SUP], Beate Preu?[SUP] 5 [/SUP], Ilona Hagelstein[SUP] 1 3 [/SUP], Melanie M?rklin[SUP] 1 3 [/SUP], Tamam Bakchoul[SUP] 17 [/SUP], C?cile Gouttefangeas[SUP] 2 3 4 [/SUP], Oliver Kohlbacher[SUP] 16 18 19 20 [/SUP], Reinhild Klein[SUP] 5 [/SUP], Stefan Stevanović[SUP] 2 4 [/SUP], Hans-Georg Rammensee[SUP] 2 3 4 [/SUP], Juliane S Walz[SUP] 21 22 23 [/SUP]
Affiliations
- PMID: 32999467
- DOI: 10.1038/s41590-020-00808-x
Abstract
T cell immunity is central for the control of viral infections. To characterize T cell immunity, but also for the development of vaccines, identification of exact viral T cell epitopes is fundamental. Here we identify and characterize multiple dominant and subdominant SARS-CoV-2 HLA class I and HLA-DR peptides as potential T cell epitopes in COVID-19 convalescent and unexposed individuals. SARS-CoV-2-specific peptides enabled detection of post-infectious T cell immunity, even in seronegative convalescent individuals. Cross-reactive SARS-CoV-2 peptides revealed pre-existing T cell responses in 81% of unexposed individuals and validated similarity with common cold coronaviruses, providing a functional basis for heterologous immunity in SARS-CoV-2 infection. Diversity of SARS-CoV-2 T cell responses was associated with mild symptoms of COVID-19, providing evidence that immunity requires recognition of multiple epitopes. Together, the proposed SARS-CoV-2 T cell epitopes enable identification of heterologous and post-infectious T cell immunity and facilitate development of diagnostic, preventive and therapeutic measures for COVID-19.