tetano
Editor, Senior Moderator
Nat Microbiol
. 2023 Sep 25.
doi: 10.1038/s41564-023-01476-x. Online ahead of print. Host range, transmissibility and antigenicity of a pangolin coronavirus
Yixuan J Hou[SUP] 1 2 [/SUP], Shiho Chiba[SUP] 3 [/SUP], Sarah R Leist[SUP] 1 [/SUP], Rita M Meganck[SUP] 1 [/SUP], David R Martinez[SUP] 1 [/SUP], Alexandra Schäfer[SUP] 1 [/SUP], Nicholas J Catanzaro[SUP] 1 [/SUP], Vishwaraj Sontake[SUP] 4 [/SUP], Ande West[SUP] 1 [/SUP], Catlin E Edwards[SUP] 1 [/SUP], Boyd Yount[SUP] 1 [/SUP], Rhianna E Lee[SUP] 5 [/SUP], Samuel C Gallant[SUP] 5 [/SUP], Seth J Zost[SUP] 6 [/SUP], John Powers[SUP] 1 [/SUP], Lily Adams[SUP] 1 [/SUP], Edgar F Kong[SUP] 1 [/SUP], Melissa Mattocks[SUP] 1 [/SUP], Aleksandra Tata[SUP] 4 [/SUP], Scott H Randell[SUP] 5 [/SUP], Purushothama R Tata[SUP] 4 [/SUP], Peter Halfmann[SUP] 3 [/SUP], James E Crowe Jr[SUP] 6 [/SUP], Yoshihiro Kawaoka[SUP] 3 7 [/SUP], Ralph S Baric[SUP] 8 9 [/SUP]
Affiliations
The pathogenic and cross-species transmission potential of SARS-CoV-2-related coronaviruses (CoVs) remain poorly characterized. Here we recovered a wild-type pangolin (Pg) CoV GD strain including derivatives encoding reporter genes using reverse genetics. In primary human cells, PgCoV replicated efficiently but with reduced fitness and showed less efficient transmission via airborne route compared with SARS-CoV-2 in hamsters. PgCoV was potently inhibited by US Food and Drug Administration approved drugs, and neutralized by COVID-19 patient sera and SARS-CoV-2 therapeutic antibodies in vitro. A pan-Sarbecovirus antibody and SARS-CoV-2 S2P recombinant protein vaccine protected BALB/c mice from PgCoV infection. In K18-hACE2 mice, PgCoV infection caused severe clinical disease, but mice were protected by a SARS-CoV-2 human antibody. Efficient PgCoV replication in primary human cells and hACE2 mice, coupled with a capacity for airborne spread, highlights an emergence potential. However, low competitive fitness, pre-immune humans and the benefit of COVID-19 countermeasures should impede its ability to spread globally in human populations.
. 2023 Sep 25.
doi: 10.1038/s41564-023-01476-x. Online ahead of print. Host range, transmissibility and antigenicity of a pangolin coronavirus
Yixuan J Hou[SUP] 1 2 [/SUP], Shiho Chiba[SUP] 3 [/SUP], Sarah R Leist[SUP] 1 [/SUP], Rita M Meganck[SUP] 1 [/SUP], David R Martinez[SUP] 1 [/SUP], Alexandra Schäfer[SUP] 1 [/SUP], Nicholas J Catanzaro[SUP] 1 [/SUP], Vishwaraj Sontake[SUP] 4 [/SUP], Ande West[SUP] 1 [/SUP], Catlin E Edwards[SUP] 1 [/SUP], Boyd Yount[SUP] 1 [/SUP], Rhianna E Lee[SUP] 5 [/SUP], Samuel C Gallant[SUP] 5 [/SUP], Seth J Zost[SUP] 6 [/SUP], John Powers[SUP] 1 [/SUP], Lily Adams[SUP] 1 [/SUP], Edgar F Kong[SUP] 1 [/SUP], Melissa Mattocks[SUP] 1 [/SUP], Aleksandra Tata[SUP] 4 [/SUP], Scott H Randell[SUP] 5 [/SUP], Purushothama R Tata[SUP] 4 [/SUP], Peter Halfmann[SUP] 3 [/SUP], James E Crowe Jr[SUP] 6 [/SUP], Yoshihiro Kawaoka[SUP] 3 7 [/SUP], Ralph S Baric[SUP] 8 9 [/SUP]
Affiliations
- PMID: 37749254
- DOI: 10.1038/s41564-023-01476-x
The pathogenic and cross-species transmission potential of SARS-CoV-2-related coronaviruses (CoVs) remain poorly characterized. Here we recovered a wild-type pangolin (Pg) CoV GD strain including derivatives encoding reporter genes using reverse genetics. In primary human cells, PgCoV replicated efficiently but with reduced fitness and showed less efficient transmission via airborne route compared with SARS-CoV-2 in hamsters. PgCoV was potently inhibited by US Food and Drug Administration approved drugs, and neutralized by COVID-19 patient sera and SARS-CoV-2 therapeutic antibodies in vitro. A pan-Sarbecovirus antibody and SARS-CoV-2 S2P recombinant protein vaccine protected BALB/c mice from PgCoV infection. In K18-hACE2 mice, PgCoV infection caused severe clinical disease, but mice were protected by a SARS-CoV-2 human antibody. Efficient PgCoV replication in primary human cells and hACE2 mice, coupled with a capacity for airborne spread, highlights an emergence potential. However, low competitive fitness, pre-immune humans and the benefit of COVID-19 countermeasures should impede its ability to spread globally in human populations.