tetano
Editor, Senior Moderator
Nature Immunology | Resource
Systems biology of vaccination for seasonal influenza in humans
Helder I Nakaya,
Jens Wrammert,
Eva K Lee,
Luigi Racioppi,
Stephanie Marie-Kunze,
W Nicholas Haining,
Anthony R Means,
Sudhir P Kasturi,
Nooruddin Khan,
Gui-Mei Li,
Megan McCausland,
Vibhu Kanchan,
Kenneth E Kokko,
Shuzhao Li,
Rivka Elbein,
Aneesh K Mehta,
Alan Aderem,
Kanta Subbarao,
Rafi Ahmed
& Bali Pulendran
Affiliations
Contributions
Corresponding author
Nature Immunology
(2011)
doi:10.1038/ni.2067
Received
12 April 2011
Accepted
06 June 2011
Published online
10 July 2011
Here we have used a systems biology approach to study innate and adaptive responses to vaccination against influenza in humans during three consecutive influenza seasons. We studied healthy adults vaccinated with trivalent inactivated influenza vaccine (TIV) or live attenuated influenza vaccine (LAIV). TIV induced higher antibody titers and more plasmablasts than LAIV did. In subjects vaccinated with TIV, early molecular signatures correlated with and could be used to accurately predict later antibody titers in two independent trials. Notably, expression of the kinase CaMKIV at day 3 was inversely correlated with later antibody titers. Vaccination of CaMKIV-deficient mice with TIV induced enhanced antigen-specific antibody titers, which demonstrated an unappreciated role for CaMKIV in the regulation of antibody responses. Thus, systems approaches can be used to predict immunogenicity and provide new mechanistic insights about vaccines.
http://www.nature.com/ni/journal/vaop/ncurrent/full/ni.2067.html
Systems biology of vaccination for seasonal influenza in humans
Helder I Nakaya,
Jens Wrammert,
Eva K Lee,
Luigi Racioppi,
Stephanie Marie-Kunze,
W Nicholas Haining,
Anthony R Means,
Sudhir P Kasturi,
Nooruddin Khan,
Gui-Mei Li,
Megan McCausland,
Vibhu Kanchan,
Kenneth E Kokko,
Shuzhao Li,
Rivka Elbein,
Aneesh K Mehta,
Alan Aderem,
Kanta Subbarao,
Rafi Ahmed
& Bali Pulendran
Affiliations
Contributions
Corresponding author
Nature Immunology
(2011)
doi:10.1038/ni.2067
Received
12 April 2011
Accepted
06 June 2011
Published online
10 July 2011
Here we have used a systems biology approach to study innate and adaptive responses to vaccination against influenza in humans during three consecutive influenza seasons. We studied healthy adults vaccinated with trivalent inactivated influenza vaccine (TIV) or live attenuated influenza vaccine (LAIV). TIV induced higher antibody titers and more plasmablasts than LAIV did. In subjects vaccinated with TIV, early molecular signatures correlated with and could be used to accurately predict later antibody titers in two independent trials. Notably, expression of the kinase CaMKIV at day 3 was inversely correlated with later antibody titers. Vaccination of CaMKIV-deficient mice with TIV induced enhanced antigen-specific antibody titers, which demonstrated an unappreciated role for CaMKIV in the regulation of antibody responses. Thus, systems approaches can be used to predict immunogenicity and provide new mechanistic insights about vaccines.
http://www.nature.com/ni/journal/vaop/ncurrent/full/ni.2067.html