tetano
Editor, Senior Moderator
PeerJ
. 2025 Jan 22:13:e18856.
doi: 10.7717/peerj.18856. eCollection 2025. Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine
Guanguan Qiu[SUP] #[/SUP][SUP] 1 [/SUP], Ruoyang Zhang[SUP] #[/SUP][SUP] 2 [/SUP], Huifeng Qian[SUP] 1 [/SUP], Ruoqiong Huang[SUP] 2 [/SUP], Jie Xia[SUP] 2 [/SUP], Ruoxi Zang[SUP] 2 [/SUP], Zhenkai Le[SUP] 2 [/SUP], Qiang Shu[SUP] 2 [/SUP], Jianguo Xu[SUP] 1 2 [/SUP], Guoping Zheng[SUP] 1 [/SUP], Jiangmei Wang[SUP] 2 [/SUP]
Affiliations
COVID-19 vaccination is the most effective strategy for preventing severe disease and death. Inactivated vaccines are the most accessible type of COVID-19 vaccines in developing countries. Several studies, including work from our group, have demonstrated that the third dose (booster vaccination) of inactivated COVID-19 vaccine induces robust humoral and cellular immune responses. The present study aimed to examine miRNA expression profile in participants who received a homologous third dose of the CoronaVac vaccine. Samples of peripheral blood mononuclear cells (PBMCs) were collected from healthcare volunteers both before and 1-2 weeks after the booster dose. miRNA microarray analysis in a discovery cohort of six volunteers identified 67 miRNAs with differential expression. Subsequently, the expression of six miRNAs related to immune responses was examined in a validation cohort of 31 participants via qRT-PCR. Our results validated the differential expression of miR-25-5p, miR-34c-3p, and miR-206 post-booster, with a significant correlation to the receptor binding domain (RBD)-specific antibody. Bioinformatic analysis suggested that miR-25-5p, miR-34c-3p, and miR-206 may target multiple pathways involved in immune regulation and inflammation. Therefore, our study highlights miR-25-5p, miR-34c-3p, and miR-206 in PBMCs as promising biomarkers for assessing the immune response induced by the booster dose of the CoronaVac vaccine.
Keywords: COVID-19; CoronaVac; Immune response; Inactivated vaccine; MicroRNA; Peripheral blood mononuclear cells.
. 2025 Jan 22:13:e18856.
doi: 10.7717/peerj.18856. eCollection 2025. Altered expression of miRNA profile in peripheral blood mononuclear cells following the third dose of inactivated COVID-19 vaccine
Guanguan Qiu[SUP] #[/SUP][SUP] 1 [/SUP], Ruoyang Zhang[SUP] #[/SUP][SUP] 2 [/SUP], Huifeng Qian[SUP] 1 [/SUP], Ruoqiong Huang[SUP] 2 [/SUP], Jie Xia[SUP] 2 [/SUP], Ruoxi Zang[SUP] 2 [/SUP], Zhenkai Le[SUP] 2 [/SUP], Qiang Shu[SUP] 2 [/SUP], Jianguo Xu[SUP] 1 2 [/SUP], Guoping Zheng[SUP] 1 [/SUP], Jiangmei Wang[SUP] 2 [/SUP]
Affiliations
- PMID: 39866557
- PMCID: PMC11760199
- DOI: 10.7717/peerj.18856
COVID-19 vaccination is the most effective strategy for preventing severe disease and death. Inactivated vaccines are the most accessible type of COVID-19 vaccines in developing countries. Several studies, including work from our group, have demonstrated that the third dose (booster vaccination) of inactivated COVID-19 vaccine induces robust humoral and cellular immune responses. The present study aimed to examine miRNA expression profile in participants who received a homologous third dose of the CoronaVac vaccine. Samples of peripheral blood mononuclear cells (PBMCs) were collected from healthcare volunteers both before and 1-2 weeks after the booster dose. miRNA microarray analysis in a discovery cohort of six volunteers identified 67 miRNAs with differential expression. Subsequently, the expression of six miRNAs related to immune responses was examined in a validation cohort of 31 participants via qRT-PCR. Our results validated the differential expression of miR-25-5p, miR-34c-3p, and miR-206 post-booster, with a significant correlation to the receptor binding domain (RBD)-specific antibody. Bioinformatic analysis suggested that miR-25-5p, miR-34c-3p, and miR-206 may target multiple pathways involved in immune regulation and inflammation. Therefore, our study highlights miR-25-5p, miR-34c-3p, and miR-206 in PBMCs as promising biomarkers for assessing the immune response induced by the booster dose of the CoronaVac vaccine.
Keywords: COVID-19; CoronaVac; Immune response; Inactivated vaccine; MicroRNA; Peripheral blood mononuclear cells.