tetano
Editor, Senior Moderator
Proc Natl Acad Sci U S A
. 2023 May 23;120(21):e2214936120.
doi: 10.1073/pnas.2214936120. Epub 2023 May 16. Influenza A virus exploits transferrin receptor recycling to enter host cells
Beryl Mazel-Sanchez[SUP] 1 [/SUP], Chengyue Niu[SUP] 1 [/SUP], Nathalia Williams[SUP] 1 [/SUP], Michael Bachmann[SUP] 2 [/SUP], Hélèna Choltus[SUP] 1 [/SUP], Filo Silva[SUP] 1 [/SUP], Véronique Serre-Beinier[SUP] 3 [/SUP], Wolfram Karenovics[SUP] 3 [/SUP], Justyna Iwaszkiewicz[SUP] 4 [/SUP], Vincent Zoete[SUP] 4 5 [/SUP], Laurent Kaiser[SUP] 1 6 7 8 [/SUP], Oliver Hartley[SUP] 9 [/SUP], Bernhard Wehrle-Haller[SUP] 2 [/SUP], Mirco Schmolke[SUP] 1 10 [/SUP]
Affiliations
Influenza A virus (IAV) enters host cells mostly through clathrin-dependent receptor-mediated endocytosis. A single bona fide entry receptor protein supporting this entry mechanism remains elusive. Here we performed proximity ligation of biotin to host cell surface proteins in the vicinity of attached trimeric hemagglutinin-HRP and characterized biotinylated targets using mass spectrometry. This approach identified transferrin receptor 1 (TfR1) as a candidate entry protein. Genetic gain-of-function and loss-of-function experiments, as well as in vitro and in vivo chemical inhibition, confirmed the functional involvement of TfR1 in IAV entry. Recycling deficient mutants of TfR1 do not support entry, indicating that TfR1 recycling is essential for this function. The binding of virions to TfR1 via sialic acids confirmed its role as a directly acting entry factor, but unexpectedly even headless TfR1 promoted IAV particle uptake in trans. TIRF microscopy localized the entering virus-like particles in the vicinity of TfR1. Our data identify TfR1 recycling as a revolving door mechanism exploited by IAV to enter host cells.
Keywords: antiviral; endocytosis; influenza A virus; recycling; transferrin receptor 1.
. 2023 May 23;120(21):e2214936120.
doi: 10.1073/pnas.2214936120. Epub 2023 May 16. Influenza A virus exploits transferrin receptor recycling to enter host cells
Beryl Mazel-Sanchez[SUP] 1 [/SUP], Chengyue Niu[SUP] 1 [/SUP], Nathalia Williams[SUP] 1 [/SUP], Michael Bachmann[SUP] 2 [/SUP], Hélèna Choltus[SUP] 1 [/SUP], Filo Silva[SUP] 1 [/SUP], Véronique Serre-Beinier[SUP] 3 [/SUP], Wolfram Karenovics[SUP] 3 [/SUP], Justyna Iwaszkiewicz[SUP] 4 [/SUP], Vincent Zoete[SUP] 4 5 [/SUP], Laurent Kaiser[SUP] 1 6 7 8 [/SUP], Oliver Hartley[SUP] 9 [/SUP], Bernhard Wehrle-Haller[SUP] 2 [/SUP], Mirco Schmolke[SUP] 1 10 [/SUP]
Affiliations
- PMID: 37192162
- DOI: 10.1073/pnas.2214936120
Influenza A virus (IAV) enters host cells mostly through clathrin-dependent receptor-mediated endocytosis. A single bona fide entry receptor protein supporting this entry mechanism remains elusive. Here we performed proximity ligation of biotin to host cell surface proteins in the vicinity of attached trimeric hemagglutinin-HRP and characterized biotinylated targets using mass spectrometry. This approach identified transferrin receptor 1 (TfR1) as a candidate entry protein. Genetic gain-of-function and loss-of-function experiments, as well as in vitro and in vivo chemical inhibition, confirmed the functional involvement of TfR1 in IAV entry. Recycling deficient mutants of TfR1 do not support entry, indicating that TfR1 recycling is essential for this function. The binding of virions to TfR1 via sialic acids confirmed its role as a directly acting entry factor, but unexpectedly even headless TfR1 promoted IAV particle uptake in trans. TIRF microscopy localized the entering virus-like particles in the vicinity of TfR1. Our data identify TfR1 recycling as a revolving door mechanism exploited by IAV to enter host cells.
Keywords: antiviral; endocytosis; influenza A virus; recycling; transferrin receptor 1.