tetano
Editor, Senior Moderator
Protein Eng Des Sel
. 2022 Feb 17;35:gzab035.
doi: 10.1093/protein/gzab035.
Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species
Pete Heinzelman[SUP] 1 [/SUP], Jonathan C Greenhalgh[SUP] 1 2 [/SUP], Philip A Romero[SUP] 1 2 [/SUP]
Affiliations
Abstract
Understanding how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interacts with different mammalian angiotensin-converting enzyme II (ACE2) cell entry receptors elucidates determinants of virus transmission and facilitates development of vaccines for humans and animals. Yeast display-based directed evolution identified conserved ACE2 mutations that increase spike binding across multiple species. Gln42Leu increased ACE2-spike binding for human and four of four other mammalian ACE2s; Leu79Ile had an effect for human and three of three mammalian ACE2s. These residues are highly represented, 83% for Gln42 and 56% for Leu79, among mammalian ACE2s. The above findings can be important in protecting humans and animals from existing and future SARS-CoV-2 variants.
Keywords: SARS-CoV-2; angiotensin-converting enzyme II; directed evolution; vaccine; yeast surface display.
. 2022 Feb 17;35:gzab035.
doi: 10.1093/protein/gzab035.
Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species
Pete Heinzelman[SUP] 1 [/SUP], Jonathan C Greenhalgh[SUP] 1 2 [/SUP], Philip A Romero[SUP] 1 2 [/SUP]
Affiliations
- PMID: 35174856
- DOI: 10.1093/protein/gzab035
Abstract
Understanding how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interacts with different mammalian angiotensin-converting enzyme II (ACE2) cell entry receptors elucidates determinants of virus transmission and facilitates development of vaccines for humans and animals. Yeast display-based directed evolution identified conserved ACE2 mutations that increase spike binding across multiple species. Gln42Leu increased ACE2-spike binding for human and four of four other mammalian ACE2s; Leu79Ile had an effect for human and three of three mammalian ACE2s. These residues are highly represented, 83% for Gln42 and 56% for Leu79, among mammalian ACE2s. The above findings can be important in protecting humans and animals from existing and future SARS-CoV-2 variants.
Keywords: SARS-CoV-2; angiotensin-converting enzyme II; directed evolution; vaccine; yeast surface display.