tetano
Editor, Senior Moderator
J Gen Virol. 2011 May 4. [Epub ahead of print]
Pulmonary pathology of pandemic influenza A/H1N1 virus (2009) infected ferrets upon longitudinal evaluation by computed tomography.
Veldhuis Kroeze E, van Amerongen G, Dijkshoorn M, Simon J, de Waal L, Hartmann I, Krestin G, Kuiken T, Osterhaus A, Stittelaar K.
Source
Viroclinics Biosciences B.V.;
Abstract
We investigated the development of pulmonary lesions in ferrets following infection with the 2009 pandemic A/H1N1 influenza virus by means of computed tomography (CT) and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT-scanning in all infected lungs corresponded to areas of alveolar edema at necropsy. These areas were most pronounced on day 3 and gradually decreased from day 4 to day 7 post-infection. This pilot study shows that non-invasive imaging procedure allows quantification and characterization of influenza induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in preclinical pharmaceutical efficacy studies.
PMID:
21543558
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21543558
Pulmonary pathology of pandemic influenza A/H1N1 virus (2009) infected ferrets upon longitudinal evaluation by computed tomography.
Veldhuis Kroeze E, van Amerongen G, Dijkshoorn M, Simon J, de Waal L, Hartmann I, Krestin G, Kuiken T, Osterhaus A, Stittelaar K.
Source
Viroclinics Biosciences B.V.;
Abstract
We investigated the development of pulmonary lesions in ferrets following infection with the 2009 pandemic A/H1N1 influenza virus by means of computed tomography (CT) and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT-scanning in all infected lungs corresponded to areas of alveolar edema at necropsy. These areas were most pronounced on day 3 and gradually decreased from day 4 to day 7 post-infection. This pilot study shows that non-invasive imaging procedure allows quantification and characterization of influenza induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in preclinical pharmaceutical efficacy studies.
PMID:
21543558
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21543558