tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2019 Dec 1. doi: 10.1111/irv.12701. [Epub ahead of print] [h=1]Rapid detection of respiratory organisms with FilmArray respiratory panel and its impact on clinical decisions in Shanghai, China, 2016-2018.[/h]
Qian Y[SUP]1[/SUP], Ai J[SUP]1[/SUP], Wu J[SUP]1[/SUP], Yu S[SUP]1[/SUP], Cui P[SUP]1[/SUP], Gao Y[SUP]1[/SUP], Jin J[SUP]1[/SUP], Weng X[SUP]1[/SUP], Zhang W[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China. 2 State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China. 3 National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, China. 4 Key Laboratory of Medical Molecular Virology (MOE/MOH) and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] In this study, we evaluated the diagnostic potential and clinical impact of an automated multiplex PCR platform (the FilmArray Respiratory Panel; FA-RP), specially designed for pathogen detection in respiratory tract infections in adults with unexplained pneumonia (UP).
[h=4]METHODS:[/h] A total of 112 UP patients in Shanghai, China, were enrolled prospectively and assessed using the FA-RP from October 2016 to March 2018. We examined the test results and their influence on clinical decisions. Furthermore, as a control group, we retrospectively obtained the clinical data of 70 UP patients between October 2014 and March 2016 (before the FA-RP was available). The two patient groups were compared with respect to factors, including general antimicrobial use and defined daily dose (DDD) numbers.
[h=4]RESULTS:[/h] Between October 2016 and March 2018, the positive rate obtained using FA-RP for UP was 76.8%. The primary pathogens in adults with UP were Influenza A/B (47.3%, 53/112). Compared with the patients before FA-RP was available, patients who underwent FA-RP testing had higher rates of antiviral drug use and antibiotic de-escalation during clinical treatment. FA-RP significantly decreased the total DDDs of antibiotic or antifungal drugs DDDs by 7 days after admission (10.6 ? 2.5 vs 14.1 ? 8.8, P < .01).
[h=4]CONCLUSIONS:[/h] The FA-RP is a rapid and sensitive nucleic acid amplification test method for UP diagnosis in adults. The application of FA-RP may lead to a more accurately targeted antimicrobial treatment and reduced use of antibiotic/antifungal drugs.
? 2019 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.
[h=4]KEYWORDS:[/h] FilmArray respiratory panel; antibiotic use; clinical decision; respiratory virus; unexplained pneumonia
PMID: 31786832 DOI: 10.1111/irv.12701
Free full text
Qian Y[SUP]1[/SUP], Ai J[SUP]1[/SUP], Wu J[SUP]1[/SUP], Yu S[SUP]1[/SUP], Cui P[SUP]1[/SUP], Gao Y[SUP]1[/SUP], Jin J[SUP]1[/SUP], Weng X[SUP]1[/SUP], Zhang W[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3,[/SUP][SUP]4[/SUP].
[h=3]Author information[/h] 1 Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China. 2 State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, China. 3 National Clinical Research Center for Aging and Medicine, Huashan Hospital, Fudan University, Shanghai, China. 4 Key Laboratory of Medical Molecular Virology (MOE/MOH) and Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] In this study, we evaluated the diagnostic potential and clinical impact of an automated multiplex PCR platform (the FilmArray Respiratory Panel; FA-RP), specially designed for pathogen detection in respiratory tract infections in adults with unexplained pneumonia (UP).
[h=4]METHODS:[/h] A total of 112 UP patients in Shanghai, China, were enrolled prospectively and assessed using the FA-RP from October 2016 to March 2018. We examined the test results and their influence on clinical decisions. Furthermore, as a control group, we retrospectively obtained the clinical data of 70 UP patients between October 2014 and March 2016 (before the FA-RP was available). The two patient groups were compared with respect to factors, including general antimicrobial use and defined daily dose (DDD) numbers.
[h=4]RESULTS:[/h] Between October 2016 and March 2018, the positive rate obtained using FA-RP for UP was 76.8%. The primary pathogens in adults with UP were Influenza A/B (47.3%, 53/112). Compared with the patients before FA-RP was available, patients who underwent FA-RP testing had higher rates of antiviral drug use and antibiotic de-escalation during clinical treatment. FA-RP significantly decreased the total DDDs of antibiotic or antifungal drugs DDDs by 7 days after admission (10.6 ? 2.5 vs 14.1 ? 8.8, P < .01).
[h=4]CONCLUSIONS:[/h] The FA-RP is a rapid and sensitive nucleic acid amplification test method for UP diagnosis in adults. The application of FA-RP may lead to a more accurately targeted antimicrobial treatment and reduced use of antibiotic/antifungal drugs.
? 2019 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.
[h=4]KEYWORDS:[/h] FilmArray respiratory panel; antibiotic use; clinical decision; respiratory virus; unexplained pneumonia
PMID: 31786832 DOI: 10.1111/irv.12701
Free full text