tetano
Editor, Senior Moderator
J Virol Methods. 2010 Sep 14. [Epub ahead of print]
Real-time reverse transcription-PCR assay for differentiating the Pandemic H1N1 influenza virus 2009 from swine influenza viruses.
Hiromoto Y, Uchida Y, Takemae N, Hayashi T, Tsuda T, Saito T.
Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba, Ibaraki 305-0856, Japan; Zoonotic Diseases Collaboration Center (ZDCC), Kasetklang, Chatuchak, Bangkok 10900, Thailand.
Abstract
Since the Pandemic H1N1 influenza virus 2009 (H1N1pdm) emerged in human in 2009, H1N1pdm, classical swine H1, Eurasian avian-like H1, human-like H1 and human-like H3 swine influenza viruses have circulated in pig populations, and avian H9N2 viruses have been isolated in pigs as well. In this study, TaqMan single-step real-time reverse transcription-PCR (rtRT-PCR) assays targeting the hemagglutinin gene were developed to differentiate H1N1pdm from other genetic lineages of the H1 subtype and other subtypes of influenza viruses circulating in human and pig populations for veterinary use. H1N1pdm rtRT-PCR detected H1N1pdm RNA and did not cross-react with classical swine H1, Eurasian avian-like H1, human-like H1, human-like H3 swine and avian H9 influenza viruses RNA. Classical swine H1, Eurasian avian-like H1, human-like H1 and H3 and avian H9 rtRT-PCR were reacted exclusively with viral RNA of their respective lineages and subtypes. The results demonstrate that these assays are useful for the diagnosis of the H1N1pdm virus in both human- and animal-health-related fields.
PMID: 20849878 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20849878
Real-time reverse transcription-PCR assay for differentiating the Pandemic H1N1 influenza virus 2009 from swine influenza viruses.
Hiromoto Y, Uchida Y, Takemae N, Hayashi T, Tsuda T, Saito T.
Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba, Ibaraki 305-0856, Japan; Zoonotic Diseases Collaboration Center (ZDCC), Kasetklang, Chatuchak, Bangkok 10900, Thailand.
Abstract
Since the Pandemic H1N1 influenza virus 2009 (H1N1pdm) emerged in human in 2009, H1N1pdm, classical swine H1, Eurasian avian-like H1, human-like H1 and human-like H3 swine influenza viruses have circulated in pig populations, and avian H9N2 viruses have been isolated in pigs as well. In this study, TaqMan single-step real-time reverse transcription-PCR (rtRT-PCR) assays targeting the hemagglutinin gene were developed to differentiate H1N1pdm from other genetic lineages of the H1 subtype and other subtypes of influenza viruses circulating in human and pig populations for veterinary use. H1N1pdm rtRT-PCR detected H1N1pdm RNA and did not cross-react with classical swine H1, Eurasian avian-like H1, human-like H1, human-like H3 swine and avian H9 influenza viruses RNA. Classical swine H1, Eurasian avian-like H1, human-like H1 and H3 and avian H9 rtRT-PCR were reacted exclusively with viral RNA of their respective lineages and subtypes. The results demonstrate that these assays are useful for the diagnosis of the H1N1pdm virus in both human- and animal-health-related fields.
PMID: 20849878 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/20849878