tetano
Editor, Senior Moderator
J Gen Virol. 2015 May 18. pii: vir.0.000190. doi: 10.1099/vir.0.000190. [Epub ahead of print]
[h=1]Replication and transmission of mammalian-adapted H9 subtype influenza virus in pigs and quail.[/h] Obadan AO[SUP]1[/SUP], Kimble BJ[SUP]1[/SUP], Rajao DS[SUP]2[/SUP], Lager K[SUP]2[/SUP], Santos JJ[SUP]1[/SUP], Vincent A[SUP]2[/SUP], Perez DR[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus is a major pathogen of birds, swine, and humans. Strains can jump between species in a process often requiring mutations and reassortment resulting in outbreaks and, potentially, pandemics. H9N2 avian influenza is predominant in poultry across Asia and occasionally infects humans and swine. Pandemic H1N1 (H1N1pdm) is endemic in humans and swine and has a history of reassortment in pigs. Previous studies have shown the compatibility of H9N2 and H1N1pdm for reassortment in ferrets, a model for human infection and transmission. Here, the effects of ferret adaptation of H9 surface gene segments on the infectivity and transmission in at-risk natural hosts, specifically swine and quail, is analyzed. Reassortant H9N1 and H9N2 viruses, carrying 7 or 6 gene segments from H1N1pdm showed infectivity and transmissibility in swine, unlike the wholly avian H9N2 virus with ferret-adapted surface genes. In quail, only the reassortant H9N2 with the 6 internal gene segments from H1N1pdm strain was able to infect and transmit, however, less efficiently than the wholly avian H9N2 virus with ferret-adapted surface genes. These results highlight that ferret-adapted mutations on the hemagglutinin of H9 subtype virus do not restrict the virus ability to infect swine or quail and that the ability to transmit in these species depend on the context of the whole virus. As such, this study emphasizes the threat H9N2 reassortant viruses pose to humans and agricultural species and the importance of a virus' genetic constellation in its ability to replicate and transmit in natural hosts of influenza.
PMID: 25986634 [PubMed - as supplied by publisher]
[h=1]Replication and transmission of mammalian-adapted H9 subtype influenza virus in pigs and quail.[/h] Obadan AO[SUP]1[/SUP], Kimble BJ[SUP]1[/SUP], Rajao DS[SUP]2[/SUP], Lager K[SUP]2[/SUP], Santos JJ[SUP]1[/SUP], Vincent A[SUP]2[/SUP], Perez DR[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus is a major pathogen of birds, swine, and humans. Strains can jump between species in a process often requiring mutations and reassortment resulting in outbreaks and, potentially, pandemics. H9N2 avian influenza is predominant in poultry across Asia and occasionally infects humans and swine. Pandemic H1N1 (H1N1pdm) is endemic in humans and swine and has a history of reassortment in pigs. Previous studies have shown the compatibility of H9N2 and H1N1pdm for reassortment in ferrets, a model for human infection and transmission. Here, the effects of ferret adaptation of H9 surface gene segments on the infectivity and transmission in at-risk natural hosts, specifically swine and quail, is analyzed. Reassortant H9N1 and H9N2 viruses, carrying 7 or 6 gene segments from H1N1pdm showed infectivity and transmissibility in swine, unlike the wholly avian H9N2 virus with ferret-adapted surface genes. In quail, only the reassortant H9N2 with the 6 internal gene segments from H1N1pdm strain was able to infect and transmit, however, less efficiently than the wholly avian H9N2 virus with ferret-adapted surface genes. These results highlight that ferret-adapted mutations on the hemagglutinin of H9 subtype virus do not restrict the virus ability to infect swine or quail and that the ability to transmit in these species depend on the context of the whole virus. As such, this study emphasizes the threat H9N2 reassortant viruses pose to humans and agricultural species and the importance of a virus' genetic constellation in its ability to replicate and transmit in natural hosts of influenza.
PMID: 25986634 [PubMed - as supplied by publisher]