tetano
Editor, Senior Moderator
Sci Adv
. 2022 Dec 14;8(50):eadc9937.
doi: 10.1126/sciadv.adc9937. Epub 2022 Dec 14.
A universal influenza mRNA vaccine candidate boosts T cell responses and reduces zoonotic influenza virus disease in ferrets
Koen van de Ven[SUP] 1 [/SUP], Josien Lanfermeijer[SUP] 1 2 [/SUP], Harry van Dijken[SUP] 1 [/SUP], Hiromi Muramatsu[SUP] 3 [/SUP], Caroline Vilas Boas de Melo[SUP] 1 [/SUP], Stefanie Lenz[SUP] 1 [/SUP], Florence Peters[SUP] 1 [/SUP], Mitchell B Beattie[SUP] 4 [/SUP], Paulo J C Lin[SUP] 4 [/SUP], José A Ferreira[SUP] 5 [/SUP], Judith van den Brand[SUP] 6 [/SUP], Debbie van Baarle[SUP] 1 7 [/SUP], Norbert Pardi[SUP] 3 [/SUP], Jørgen de Jonge[SUP] 1 [/SUP]
Affiliations
Abstract
Universal influenza vaccines should protect against continuously evolving and newly emerging influenza viruses. T cells may be an essential target of such vaccines, as they can clear infected cells through recognition of conserved influenza virus epitopes. We evaluated a novel T cell-inducing nucleoside-modified messenger RNA (mRNA) vaccine that encodes the conserved nucleoprotein, matrix protein 1, and polymerase basic protein 1 of an H1N1 influenza virus. To mimic the human situation, we applied the mRNA vaccine as a prime-boost regimen in naïve ferrets (mimicking young children) and as a booster in influenza-experienced ferrets (mimicking adults). The vaccine induced and boosted broadly reactive T cells in the circulation, bone marrow, and respiratory tract. Booster vaccination enhanced protection against heterosubtypic infection with a potential pandemic H7N9 influenza virus in influenza-experienced ferrets. Our findings show that mRNA vaccines encoding internal influenza virus proteins represent a promising strategy to induce broadly protective T cell immunity against influenza viruses.
. 2022 Dec 14;8(50):eadc9937.
doi: 10.1126/sciadv.adc9937. Epub 2022 Dec 14.
A universal influenza mRNA vaccine candidate boosts T cell responses and reduces zoonotic influenza virus disease in ferrets
Koen van de Ven[SUP] 1 [/SUP], Josien Lanfermeijer[SUP] 1 2 [/SUP], Harry van Dijken[SUP] 1 [/SUP], Hiromi Muramatsu[SUP] 3 [/SUP], Caroline Vilas Boas de Melo[SUP] 1 [/SUP], Stefanie Lenz[SUP] 1 [/SUP], Florence Peters[SUP] 1 [/SUP], Mitchell B Beattie[SUP] 4 [/SUP], Paulo J C Lin[SUP] 4 [/SUP], José A Ferreira[SUP] 5 [/SUP], Judith van den Brand[SUP] 6 [/SUP], Debbie van Baarle[SUP] 1 7 [/SUP], Norbert Pardi[SUP] 3 [/SUP], Jørgen de Jonge[SUP] 1 [/SUP]
Affiliations
- PMID: 36516261
- DOI: 10.1126/sciadv.adc9937
Abstract
Universal influenza vaccines should protect against continuously evolving and newly emerging influenza viruses. T cells may be an essential target of such vaccines, as they can clear infected cells through recognition of conserved influenza virus epitopes. We evaluated a novel T cell-inducing nucleoside-modified messenger RNA (mRNA) vaccine that encodes the conserved nucleoprotein, matrix protein 1, and polymerase basic protein 1 of an H1N1 influenza virus. To mimic the human situation, we applied the mRNA vaccine as a prime-boost regimen in naïve ferrets (mimicking young children) and as a booster in influenza-experienced ferrets (mimicking adults). The vaccine induced and boosted broadly reactive T cells in the circulation, bone marrow, and respiratory tract. Booster vaccination enhanced protection against heterosubtypic infection with a potential pandemic H7N9 influenza virus in influenza-experienced ferrets. Our findings show that mRNA vaccines encoding internal influenza virus proteins represent a promising strategy to induce broadly protective T cell immunity against influenza viruses.