tetano
Editor, Senior Moderator
Sci Rep
. 2021 Jun 3;11(1):11773.
doi: 10.1038/s41598-021-91264-6.
Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
Federico Colombo[SUP] 1 [/SUP], Elisa Corsiero[SUP] 2 [/SUP], Myles J Lewis[SUP] 3 [/SUP], Costantino Pitzalis[SUP] 3 [/SUP]
Affiliations
Abstract
Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in human SARS-CoV-2 genomes and (2) comparing them with other recombinant SARS-CoV-like virus created for experimental purposes. Finally, we propose a novel approach consisting in the generation of a restriction endonucleases site map of SARS-CoV-2 and other related coronavirus genomes to be used as a fingerprint to trace the virus evolution.
. 2021 Jun 3;11(1):11773.
doi: 10.1038/s41598-021-91264-6.
Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
Federico Colombo[SUP] 1 [/SUP], Elisa Corsiero[SUP] 2 [/SUP], Myles J Lewis[SUP] 3 [/SUP], Costantino Pitzalis[SUP] 3 [/SUP]
Affiliations
- PMID: 34083682
- DOI: 10.1038/s41598-021-91264-6
Abstract
Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in human SARS-CoV-2 genomes and (2) comparing them with other recombinant SARS-CoV-like virus created for experimental purposes. Finally, we propose a novel approach consisting in the generation of a restriction endonucleases site map of SARS-CoV-2 and other related coronavirus genomes to be used as a fingerprint to trace the virus evolution.