tetano
Editor, Senior Moderator
Sci Rep
. 2022 Mar 30;12(1):5375.
doi: 10.1038/s41598-022-09306-6.
Human liver organoid derived intra-hepatic bile duct cells support SARS-CoV-2 infection and replication
Vincent Chi-Hang Lui[SUP] #[/SUP][SUP] 1 [/SUP], Kenrie Pui-Yan Hui[SUP] #[/SUP][SUP] 2 [/SUP], Rosanna Ottakandathil Babu[SUP] 1 3 [/SUP], Haibing Yue[SUP] 1 [/SUP], Patrick Ho-Yu Chung[SUP] 1 [/SUP], Paul Kwong-Hang Tam[SUP] 1 4 [/SUP], Michael Chi-Wai Chan[SUP] 5 [/SUP], Kenneth Kak-Yuen Wong[SUP] 6 [/SUP]
Affiliations
Abstract
Although the main route of infection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the respiratory tract, liver injury is also commonly seen in many patients, as evidenced by deranged parenchymal liver enzymes. Furthermore, the severity of liver damage has been shown to correlate with higher mortality. Overall, the mechanism behind the liver injury remains unclear. We showed in this study that intra-hepatic bile duct cells could be grown using a human liver organoid platform. The cholangiocytes were not only susceptible to SARS-CoV-2 infection, they also supported efficient viral replication. We also showed that SARS-CoV-2 replication was much higher than SARS-CoV. Our findings suggested direct cytopathic viral damage being a mechanism for SARS-CoV-2 liver injury.
. 2022 Mar 30;12(1):5375.
doi: 10.1038/s41598-022-09306-6.
Human liver organoid derived intra-hepatic bile duct cells support SARS-CoV-2 infection and replication
Vincent Chi-Hang Lui[SUP] #[/SUP][SUP] 1 [/SUP], Kenrie Pui-Yan Hui[SUP] #[/SUP][SUP] 2 [/SUP], Rosanna Ottakandathil Babu[SUP] 1 3 [/SUP], Haibing Yue[SUP] 1 [/SUP], Patrick Ho-Yu Chung[SUP] 1 [/SUP], Paul Kwong-Hang Tam[SUP] 1 4 [/SUP], Michael Chi-Wai Chan[SUP] 5 [/SUP], Kenneth Kak-Yuen Wong[SUP] 6 [/SUP]
Affiliations
- PMID: 35354880
- DOI: 10.1038/s41598-022-09306-6
Abstract
Although the main route of infection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the respiratory tract, liver injury is also commonly seen in many patients, as evidenced by deranged parenchymal liver enzymes. Furthermore, the severity of liver damage has been shown to correlate with higher mortality. Overall, the mechanism behind the liver injury remains unclear. We showed in this study that intra-hepatic bile duct cells could be grown using a human liver organoid platform. The cholangiocytes were not only susceptible to SARS-CoV-2 infection, they also supported efficient viral replication. We also showed that SARS-CoV-2 replication was much higher than SARS-CoV. Our findings suggested direct cytopathic viral damage being a mechanism for SARS-CoV-2 liver injury.