tetano
Editor, Senior Moderator
Sci Rep
. 2024 Jan 17;14(1):1455.
doi: 10.1038/s41598-024-51665-9. Longitudinal analysis of influenza vaccination implicates regulation of RIG-I signaling by DNA methylation
Hongxiang Fu[SUP] 1 [/SUP], Harry Pickering[SUP] 2 [/SUP], Liudmilla Rubbi[SUP] 1 [/SUP], Ted M Ross[SUP] 3 4 [/SUP], Elaine F Reed[SUP] 2 [/SUP], Matteo Pellegrini[SUP] 5 [/SUP]
Affiliations
Influenza virus infection alters the promoter DNA methylation of key immune response-related genes, including type-1 interferons and proinflammatory cytokines. However, less is known about the effect of the influenza vaccine on the epigenome. We utilized a targeted DNA methylation approach to study the longitudinal effects (day 0 pre-vaccination and day 28 post-vaccination) on influenza vaccination responses in peripheral blood mononuclear cells. We found that baseline, pre-vaccination methylation profiles are associated with pre-existing, protective serological immunity. Additionally, we identified 481 sites that were differentially methylated between baseline and day 28 post-vaccination. These were enriched for genes involved in the regulation of the RIG-I signaling pathway, an important regulator of viral responses. Our results suggest that DNA methylation changes to components of the RIG-I pathway are associated with vaccine effectiveness. Therefore, immunization strategies that target this pathway may improve serological responses to influenza vaccination.
. 2024 Jan 17;14(1):1455.
doi: 10.1038/s41598-024-51665-9. Longitudinal analysis of influenza vaccination implicates regulation of RIG-I signaling by DNA methylation
Hongxiang Fu[SUP] 1 [/SUP], Harry Pickering[SUP] 2 [/SUP], Liudmilla Rubbi[SUP] 1 [/SUP], Ted M Ross[SUP] 3 4 [/SUP], Elaine F Reed[SUP] 2 [/SUP], Matteo Pellegrini[SUP] 5 [/SUP]
Affiliations
- PMID: 38228690
- DOI: 10.1038/s41598-024-51665-9
Influenza virus infection alters the promoter DNA methylation of key immune response-related genes, including type-1 interferons and proinflammatory cytokines. However, less is known about the effect of the influenza vaccine on the epigenome. We utilized a targeted DNA methylation approach to study the longitudinal effects (day 0 pre-vaccination and day 28 post-vaccination) on influenza vaccination responses in peripheral blood mononuclear cells. We found that baseline, pre-vaccination methylation profiles are associated with pre-existing, protective serological immunity. Additionally, we identified 481 sites that were differentially methylated between baseline and day 28 post-vaccination. These were enriched for genes involved in the regulation of the RIG-I signaling pathway, an important regulator of viral responses. Our results suggest that DNA methylation changes to components of the RIG-I pathway are associated with vaccine effectiveness. Therefore, immunization strategies that target this pathway may improve serological responses to influenza vaccination.