tetano
Editor, Senior Moderator
Sci Rep
. 2022 Feb 18;12(1):2356.
doi: 10.1038/s41598-022-06393-3.
Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept
Emilio Gomez-Gonzalez[SUP] 1 2 [/SUP], Alejandro Barriga-Rivera[SUP] 3 4 [/SUP], Beatriz Fernandez-Muñoz[SUP] 5 [/SUP], Jose Manuel Navas-Garcia[SUP] 6 [/SUP], Isabel Fernandez-Lizaranzu[SUP] 3 7 [/SUP], Francisco Javier Munoz-Gonzalez[SUP] 3 [/SUP], Ruben Parrilla-Giraldez[SUP] 8 [/SUP], Desiree Requena-Lancharro[SUP] 3 [/SUP], Pedro Gil-Gamboa[SUP] 3 [/SUP], Cristina Rosell-Valle[SUP] 7 5 [/SUP], Carmen Gomez-Gonzalez[SUP] 9 10 [/SUP], Maria Jose Mayorga-Buiza[SUP] 7 11 12 [/SUP], Maria Martin-Lopez[SUP] 7 5 [/SUP], Olga Muñoz[SUP] 13 [/SUP], Juan Carlos Gomez-Martin[SUP] 13 [/SUP], Maria Isabel Relimpio-Lopez[SUP] 12 14 15 [/SUP], Jesus Aceituno-Castro[SUP] 13 16 [/SUP], Manuel A Perales-Esteve[SUP] 17 [/SUP], Antonio Puppo-Moreno[SUP] 9 10 [/SUP], Francisco Jose Garcia-Cozar[SUP] 18 [/SUP], Lucia Olvera-Collantes[SUP] 19 [/SUP], Raquel Gomez-Diaz[SUP] 7 [/SUP], Silvia de Los Santos-Trigo[SUP] 20 [/SUP], Monserrat Huguet-Carrasco[SUP] 21 [/SUP], Manuel Rey[SUP] 22 [/SUP], Emilia Gomez[SUP] 23 [/SUP], Rosario Sanchez-Pernaute[SUP] 5 [/SUP], Javier Padillo-Ruiz[SUP] 7 12 24 [/SUP], Javier Marquez-Rivas[SUP] 7 12 25 26 [/SUP]
Affiliations
Abstract
Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 µL fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings.
. 2022 Feb 18;12(1):2356.
doi: 10.1038/s41598-022-06393-3.
Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept
Emilio Gomez-Gonzalez[SUP] 1 2 [/SUP], Alejandro Barriga-Rivera[SUP] 3 4 [/SUP], Beatriz Fernandez-Muñoz[SUP] 5 [/SUP], Jose Manuel Navas-Garcia[SUP] 6 [/SUP], Isabel Fernandez-Lizaranzu[SUP] 3 7 [/SUP], Francisco Javier Munoz-Gonzalez[SUP] 3 [/SUP], Ruben Parrilla-Giraldez[SUP] 8 [/SUP], Desiree Requena-Lancharro[SUP] 3 [/SUP], Pedro Gil-Gamboa[SUP] 3 [/SUP], Cristina Rosell-Valle[SUP] 7 5 [/SUP], Carmen Gomez-Gonzalez[SUP] 9 10 [/SUP], Maria Jose Mayorga-Buiza[SUP] 7 11 12 [/SUP], Maria Martin-Lopez[SUP] 7 5 [/SUP], Olga Muñoz[SUP] 13 [/SUP], Juan Carlos Gomez-Martin[SUP] 13 [/SUP], Maria Isabel Relimpio-Lopez[SUP] 12 14 15 [/SUP], Jesus Aceituno-Castro[SUP] 13 16 [/SUP], Manuel A Perales-Esteve[SUP] 17 [/SUP], Antonio Puppo-Moreno[SUP] 9 10 [/SUP], Francisco Jose Garcia-Cozar[SUP] 18 [/SUP], Lucia Olvera-Collantes[SUP] 19 [/SUP], Raquel Gomez-Diaz[SUP] 7 [/SUP], Silvia de Los Santos-Trigo[SUP] 20 [/SUP], Monserrat Huguet-Carrasco[SUP] 21 [/SUP], Manuel Rey[SUP] 22 [/SUP], Emilia Gomez[SUP] 23 [/SUP], Rosario Sanchez-Pernaute[SUP] 5 [/SUP], Javier Padillo-Ruiz[SUP] 7 12 24 [/SUP], Javier Marquez-Rivas[SUP] 7 12 25 26 [/SUP]
Affiliations
- PMID: 35181702
- DOI: 10.1038/s41598-022-06393-3
Abstract
Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 µL fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings.