tetano
Editor, Senior Moderator
Science DOI: 10.1126/science.1222908
Report
Highly Conserved Protective Epitopes on Influenza B Viruses
Cyrille Dreyfus1,*,
Nick S. Laursen1,2,*,
Ted Kwaks3,
David Zuijdgeest3,
Reza Khayat1,
Damian C. Ekiert1,?,
Jeong Hyun Lee1,
Zoltan Metlagel1,?,
Miriam V. Bujny3,
Mandy Jongeneelen3,
Remko van der Vlugt3,
Mohammed Lamrani3,
Hans J. W. M. Korse3,
Eric Geelen3,
?zcan Sahin3,
Martijn Sieuwerts3,
Just P. J. Brakenhoff3,
Ronald Vogels3,
Olive T. W. Li4,
Leo L. M. Poon4,
Malik Peiris4,
Wouter Koudstaal3,
Andrew B. Ward1,
Ian A. Wilson1,5,?,
Jaap Goudsmit3,?,
Robert H. E. Friesen3
+ Author Affiliations
1Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
2Department of Molecular Biology, Gustav Wieds Vej 10C, Aarhus 8000, Denmark.
3Crucell Vaccine Institute, Janssen Center of Excellence for Immunoprophylaxis, Archimedesweg 4-6, 2301 CA Leiden, Netherlands.
4State Key Laboratory of Emerging Infectious Diseases and School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, China.
5Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
+ Author Notes
↵? Present address: Department of Microbiology and Immunology, University of California?San Francisco, 600 16th Street, San Francisco, CA 94143, USA.
↵? Present address: Lawrence Berkeley National Laboratory, Department of Bioenergy/GTL and Structural Biology, Berkeley, CA 94720, USA.
↵?To whom correspondence should be addressed. E-mail: wilson@scripps.edu (I.A.W.); jaap.goudsmit@crucell.com (J.G.)
↵* These authors contributed equally to this work.
Abstract
Identification of broadly neutralizing antibodies against influenza A viruses has raised hopes for the development of monoclonal antibody?based immunotherapy and ?universal? vaccines for influenza. However, a significant part of the annual flu burden is caused by two cocirculating, antigenically distinct lineages of influenza B viruses. Here, we report human monoclonal antibodies, CR8033, CR8071, and CR9114, that protect mice against lethal challenge from both lineages. Antibodies CR8033 and CR8071 recognize distinct conserved epitopes in the head region of the influenza B hemagglutinin (HA), whereas CR9114 binds a conserved epitope in the HA stem and protects against lethal challenge with influenza A and B viruses. These antibodies may inform on development of monoclonal antibody?based treatments and a universal flu vaccine for all influenza A and B viruses.
http://www.sciencemag.org/content/early/2012/08/08/science.1222908
Report
Highly Conserved Protective Epitopes on Influenza B Viruses
Cyrille Dreyfus1,*,
Nick S. Laursen1,2,*,
Ted Kwaks3,
David Zuijdgeest3,
Reza Khayat1,
Damian C. Ekiert1,?,
Jeong Hyun Lee1,
Zoltan Metlagel1,?,
Miriam V. Bujny3,
Mandy Jongeneelen3,
Remko van der Vlugt3,
Mohammed Lamrani3,
Hans J. W. M. Korse3,
Eric Geelen3,
?zcan Sahin3,
Martijn Sieuwerts3,
Just P. J. Brakenhoff3,
Ronald Vogels3,
Olive T. W. Li4,
Leo L. M. Poon4,
Malik Peiris4,
Wouter Koudstaal3,
Andrew B. Ward1,
Ian A. Wilson1,5,?,
Jaap Goudsmit3,?,
Robert H. E. Friesen3
+ Author Affiliations
1Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
2Department of Molecular Biology, Gustav Wieds Vej 10C, Aarhus 8000, Denmark.
3Crucell Vaccine Institute, Janssen Center of Excellence for Immunoprophylaxis, Archimedesweg 4-6, 2301 CA Leiden, Netherlands.
4State Key Laboratory of Emerging Infectious Diseases and School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong, China.
5Skaggs Institute for Chemical Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
+ Author Notes
↵? Present address: Department of Microbiology and Immunology, University of California?San Francisco, 600 16th Street, San Francisco, CA 94143, USA.
↵? Present address: Lawrence Berkeley National Laboratory, Department of Bioenergy/GTL and Structural Biology, Berkeley, CA 94720, USA.
↵?To whom correspondence should be addressed. E-mail: wilson@scripps.edu (I.A.W.); jaap.goudsmit@crucell.com (J.G.)
↵* These authors contributed equally to this work.
Abstract
Identification of broadly neutralizing antibodies against influenza A viruses has raised hopes for the development of monoclonal antibody?based immunotherapy and ?universal? vaccines for influenza. However, a significant part of the annual flu burden is caused by two cocirculating, antigenically distinct lineages of influenza B viruses. Here, we report human monoclonal antibodies, CR8033, CR8071, and CR9114, that protect mice against lethal challenge from both lineages. Antibodies CR8033 and CR8071 recognize distinct conserved epitopes in the head region of the influenza B hemagglutinin (HA), whereas CR9114 binds a conserved epitope in the HA stem and protects against lethal challenge with influenza A and B viruses. These antibodies may inform on development of monoclonal antibody?based treatments and a universal flu vaccine for all influenza A and B viruses.
http://www.sciencemag.org/content/early/2012/08/08/science.1222908