tetano
Editor, Senior Moderator
Biochem Biophys Res Commun. 2013 Nov 19. pii: S0006-291X(13)01923-2. doi: 10.1016/j.bbrc.2013.11.041. [Epub ahead of print]
Selection of DNA aptamers that bind to influenza A viruses with high affinity and broad subtype specificity.
Shiratori I, Akitomi J, Boltz DA, Horii K, Furuichi M, Waga I.
Source
VALWAY Technology Center, NEC Soft, Ltd., 1-18-7, Shinkiba Koto-ku, Tokyo 136-8627, Japan. Electronic address: shiratori-ikuo@mxh.nes.nec.co.jp.
Abstract
Many cases of influenza are reported worldwide every year. The influenza virus often acquires new antigenicity, which is known as antigenic shift; this results in the emergence of new virus strains, for which preexisting immunity is not found in the population resulting in influenza pandemics. In the event a new strain emerges, diagnostic tools must be developed rapidly to detect the novel influenza strain. The generation of high affinity antibodies is costly and takes time; therefore, an alternative detection system, aptamer detection, provides a viable alternative to antibodies as a diagnostic tool. In this study, we developed DNA aptamers that bind to HA1 proteins of multiple influenza A virus subtypes by the SELEX procedure. To evaluate the binding properties of these aptamers using colorimetric methods, we developed a novel aptamer-based sandwich detection method employing our newly identified aptamers. This novel sandwich enzyme-linked aptamer assay successfully detected the H5N1, H1N1, and H3N2 subtypes of influenza A virus with almost equal sensitivities. These findings suggest that our aptamers are attractive candidates for use as simple and sensitive diagnostic tools that need sandwich system for detecting the Influenza A virus with broad subtype specificities.
Copyright ? 2013 The Authors. Published by Elsevier Inc. All rights reserved.
KEYWORDS:
AIV, Aptamer, Avian Influenza virus, DNA Enzyme-linked aptamer assay (ELAA), ELAA, HA, HEK, Hemagglutinin, Human Embryonic kidney, Influenza virus, MDCK, Madin-Darby canine kidney, NA, SELEX, SPR, enzyme-linked aptamer assay, neuraminidase, surface plasmon resonance, systematic evolution of ligands by exponential enrichment
PMID:
24269231
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24269231
Selection of DNA aptamers that bind to influenza A viruses with high affinity and broad subtype specificity.
Shiratori I, Akitomi J, Boltz DA, Horii K, Furuichi M, Waga I.
Source
VALWAY Technology Center, NEC Soft, Ltd., 1-18-7, Shinkiba Koto-ku, Tokyo 136-8627, Japan. Electronic address: shiratori-ikuo@mxh.nes.nec.co.jp.
Abstract
Many cases of influenza are reported worldwide every year. The influenza virus often acquires new antigenicity, which is known as antigenic shift; this results in the emergence of new virus strains, for which preexisting immunity is not found in the population resulting in influenza pandemics. In the event a new strain emerges, diagnostic tools must be developed rapidly to detect the novel influenza strain. The generation of high affinity antibodies is costly and takes time; therefore, an alternative detection system, aptamer detection, provides a viable alternative to antibodies as a diagnostic tool. In this study, we developed DNA aptamers that bind to HA1 proteins of multiple influenza A virus subtypes by the SELEX procedure. To evaluate the binding properties of these aptamers using colorimetric methods, we developed a novel aptamer-based sandwich detection method employing our newly identified aptamers. This novel sandwich enzyme-linked aptamer assay successfully detected the H5N1, H1N1, and H3N2 subtypes of influenza A virus with almost equal sensitivities. These findings suggest that our aptamers are attractive candidates for use as simple and sensitive diagnostic tools that need sandwich system for detecting the Influenza A virus with broad subtype specificities.
Copyright ? 2013 The Authors. Published by Elsevier Inc. All rights reserved.
KEYWORDS:
AIV, Aptamer, Avian Influenza virus, DNA Enzyme-linked aptamer assay (ELAA), ELAA, HA, HEK, Hemagglutinin, Human Embryonic kidney, Influenza virus, MDCK, Madin-Darby canine kidney, NA, SELEX, SPR, enzyme-linked aptamer assay, neuraminidase, surface plasmon resonance, systematic evolution of ligands by exponential enrichment
PMID:
24269231
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24269231