tetano
Editor, Senior Moderator
J Proteome Res. 2017 May 31. doi: 10.1021/acs.jproteome.7b00173. [Epub ahead of print]
[h=1]Serial metabolome changes in a prospective cohort of subjects with influenza viral infection and comparison with dengue fever.[/h] Cui L, Fang J, Ooi EE, Lee YH.
[h=3]Abstract[/h] Influenza virus infection (IVI) and dengue virus infection (DVI) are major public health threats. Between IVI and DVI, clinical symptoms can be overlapping yet infection-specific, but host metabolome changes are not well-described. Untargeted metabolomics and targeted oxylipinomic analyses were performed on sera serially collected at three phases of infection from a prospective cohort study of adult subjects with either H3N2 influenza infection or dengue fever. Untargeted metabolomics identified 26 differential metabolites and major perturbed pathways included purine metabolism, fatty acid biosynthesis and β-oxidation, tryptophan metabolism, phospholipid catabolism and steroid hormone pathway. Alterations in eight oxylipins were associated with the early symptomatic phase of H3N2 flu infection, were mostly arachidonic acid-derived, and enriched in the lipoxygenase pathway. There was significant overlap in metabolome profiles in both infections. However, differences specific to IVI and DVI were observed. DVI specifically attenuated metabolites including serotonin, bile acids and biliverdin. Additionally, metabolome changes were more persistent in IVI, in which metabolites such as hypoxanthine, inosine and xanthine of the purine metabolism pathway remained significantly elevated at 21-27 days after fever onset. This study revealed the dynamic metabolome changes in IVI subjects, and providing biochemical insights on host physiological similarities and differences between IVI and DVI.
PMID: 28560878 DOI: 10.1021/acs.jproteome.7b00173
[h=1]Serial metabolome changes in a prospective cohort of subjects with influenza viral infection and comparison with dengue fever.[/h] Cui L, Fang J, Ooi EE, Lee YH.
[h=3]Abstract[/h] Influenza virus infection (IVI) and dengue virus infection (DVI) are major public health threats. Between IVI and DVI, clinical symptoms can be overlapping yet infection-specific, but host metabolome changes are not well-described. Untargeted metabolomics and targeted oxylipinomic analyses were performed on sera serially collected at three phases of infection from a prospective cohort study of adult subjects with either H3N2 influenza infection or dengue fever. Untargeted metabolomics identified 26 differential metabolites and major perturbed pathways included purine metabolism, fatty acid biosynthesis and β-oxidation, tryptophan metabolism, phospholipid catabolism and steroid hormone pathway. Alterations in eight oxylipins were associated with the early symptomatic phase of H3N2 flu infection, were mostly arachidonic acid-derived, and enriched in the lipoxygenase pathway. There was significant overlap in metabolome profiles in both infections. However, differences specific to IVI and DVI were observed. DVI specifically attenuated metabolites including serotonin, bile acids and biliverdin. Additionally, metabolome changes were more persistent in IVI, in which metabolites such as hypoxanthine, inosine and xanthine of the purine metabolism pathway remained significantly elevated at 21-27 days after fever onset. This study revealed the dynamic metabolome changes in IVI subjects, and providing biochemical insights on host physiological similarities and differences between IVI and DVI.
PMID: 28560878 DOI: 10.1021/acs.jproteome.7b00173