Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing

[tetano]

J Infect Dev Ctries. 2011 May 28;5(5):348-52.
Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing.
Chantratita W, Sukasem C, Sirinavin S, Sankuntaw N, Srichantaratsamee C, Pasomsub E, Malathum K.
Source

Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama VI Road, Bangkok. rawct@mahidol.ac.th.
Abstract
INTRODUCTION:

We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009.
METHODOLOGY:

This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing.
RESULTS:

From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation using pyrosequencing.
CONCLUSIONS:

The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs.

PMID:
21628810
[PubMed - in process]

Free Article

http://www.ncbi.nlm.nih.gov/pubmed/21628810