tetano
Editor, Senior Moderator
Carbohydr Polym. 2016 Nov 20;153:96-104. doi: 10.1016/j.carbpol.2016.07.083. Epub 2016 Jul 20.
[h=1]Synthesis of multivalent sialyllactosamine-carrying glyco-nanoparticles with high affinity to the human influenza virus hemagglutinin.[/h] Ogata M[SUP]1[/SUP], Umemura S[SUP]2[/SUP], Sugiyama N[SUP]2[/SUP], Kuwano N[SUP]3[/SUP], Koizumi A[SUP]3[/SUP], Sawada T[SUP]4[/SUP], Yanase M[SUP]5[/SUP], Takaha T[SUP]5[/SUP], Kadokawa J[SUP]6[/SUP], Usui T[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A series of multivalent sialoglyco-conjugated nanoparticles were efficiently synthesized by using highly-branched α-glucuronic acid-linked cyclic dextrins (GlcA-HBCD) as a backbone. The sialoglycoside-moieties, with varying degrees of substitution, could be incorporated onto the preformed nanoparticles. These synthesized particles, which are highly soluble in aqueous solution, were shown to have a spherical nanostructure with a diameter of approximately 15nm. The interactions of the sialoglyco-nanoparticles (Neu5Acα2,6LacNAc-GlcA-HBCDs) with human influenza virus strain A/Beijing/262/95 (H1N1) were investigated using a hemagglutination inhibition assay. The sialoglyco-nanoparticle, in which the number of sialic acid substitution is 30, acted as a powerful inhibitor of virus binding activity. We show that both distance and multiplicity of effective ligand-virus formation play important roles in enhancing viral inhibition. Our results indicate that the GlcA-HBCD backbone can be used as a novel spherical nanocluster material for preparing a variety of glyco-nanoparticles to facilitate molecular recognition.
Copyright ? 2016 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Carbohydrate; Cluster effect; Influenza virus; Multivalency; Nanoparticle
PMID: 27561476 DOI: 10.1016/j.carbpol.2016.07.083
[PubMed - in process]
[h=1]Synthesis of multivalent sialyllactosamine-carrying glyco-nanoparticles with high affinity to the human influenza virus hemagglutinin.[/h] Ogata M[SUP]1[/SUP], Umemura S[SUP]2[/SUP], Sugiyama N[SUP]2[/SUP], Kuwano N[SUP]3[/SUP], Koizumi A[SUP]3[/SUP], Sawada T[SUP]4[/SUP], Yanase M[SUP]5[/SUP], Takaha T[SUP]5[/SUP], Kadokawa J[SUP]6[/SUP], Usui T[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] A series of multivalent sialoglyco-conjugated nanoparticles were efficiently synthesized by using highly-branched α-glucuronic acid-linked cyclic dextrins (GlcA-HBCD) as a backbone. The sialoglycoside-moieties, with varying degrees of substitution, could be incorporated onto the preformed nanoparticles. These synthesized particles, which are highly soluble in aqueous solution, were shown to have a spherical nanostructure with a diameter of approximately 15nm. The interactions of the sialoglyco-nanoparticles (Neu5Acα2,6LacNAc-GlcA-HBCDs) with human influenza virus strain A/Beijing/262/95 (H1N1) were investigated using a hemagglutination inhibition assay. The sialoglyco-nanoparticle, in which the number of sialic acid substitution is 30, acted as a powerful inhibitor of virus binding activity. We show that both distance and multiplicity of effective ligand-virus formation play important roles in enhancing viral inhibition. Our results indicate that the GlcA-HBCD backbone can be used as a novel spherical nanocluster material for preparing a variety of glyco-nanoparticles to facilitate molecular recognition.
Copyright ? 2016 Elsevier Ltd. All rights reserved.
[h=4]KEYWORDS:[/h] Carbohydrate; Cluster effect; Influenza virus; Multivalency; Nanoparticle
PMID: 27561476 DOI: 10.1016/j.carbpol.2016.07.083
[PubMed - in process]