tetano
Editor, Senior Moderator
Pediatr Infect Dis J. 2012 Mar 29. [Epub ahead of print]
T-Cell Responses in Children to Internal Influenza Antigens, 1 Year after Immunization with Pandemic H1N1 Influenza Vaccine, and Response to Revaccination with Seasonal Trivalent Inactivated Influenza Vaccine.
Lambe T, Spencer AJ, Mullarkey CE, Antrobus RD, Yu LM, de Whalley P, Thompson BA, Jones C, Chalk J, Kerridge S, Hill AV, Snape MD, Pollard AJ, Gilbert SC.
Source
1 Jenner Institute, Old Road Campus Research Building, Oxford, OX3 7DQ, UK 2 Centre for Statistics in Medicine, Wolfson College Annexe, Linton Road Oxford OX2 6UD 3 Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford OX3 7LJ.
Abstract
BACKGROUND.: During seasonal influenza epidemics, 5-15% of the population are affected with an illness having a non-trivial mortality, morbidity and economic burden. Inactivated influenza vaccines are routinely used to prevent influenza infection, primarily by inducing humoral immunity. In addition, trivalent inactivated influenza vaccines have previously been shown to boost influenza-specific T-cell responses in a small percentage of adults. We investigate here the influenza-specific T-cell response, in children, one year after pandemic H1N1 vaccination and the ability to boost the T-cell response with trivalent inactivated influenza immunization. METHODS.: TPeripheral blood mononuclear cells (PBMCs) were isolated from children previously vaccinated with pandemic H1N1 vaccine, pre & post seasonal 2010-2011 TIV vaccination. Samples were analyzed by IFN-γ ELISpot for reactogencity toward internal influenza antigens (Nucleprotein (NP), Matrix protein 1 (M1), Nonstructural Protein 1 (NS1)). RESULTS.: Basal ex vivo T-cell responses to NP, M1 & NS1 measured by IFN-γ ELISpot assay were significantly higher in those children who had previously received an AS03B-adjuvanted split virion pandemic vaccine 12 months earlier rather than a non-adjuvanted whole virion vaccine. Boosting of these responses, 21 days after 2010/2011 seasonal TIV vaccination was observed regardless of age or prior pandemic vaccination regime, although boosting was greater in those groups with the lowest initial response. CONCLUSIONS.: We show here that children previously vaccinated with the 2009 pandemic H1N1 vaccine have measurable T-cell responses 1 year post vaccination. The magnitudes of these responses are dependent on both age of vaccinee and type of pandemic H1N1 vaccine used. Following 2010/2011 seasonal TIV vaccination these T-cell responses undergo a small but significant boost.
PMID:
22466328
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22466328
T-Cell Responses in Children to Internal Influenza Antigens, 1 Year after Immunization with Pandemic H1N1 Influenza Vaccine, and Response to Revaccination with Seasonal Trivalent Inactivated Influenza Vaccine.
Lambe T, Spencer AJ, Mullarkey CE, Antrobus RD, Yu LM, de Whalley P, Thompson BA, Jones C, Chalk J, Kerridge S, Hill AV, Snape MD, Pollard AJ, Gilbert SC.
Source
1 Jenner Institute, Old Road Campus Research Building, Oxford, OX3 7DQ, UK 2 Centre for Statistics in Medicine, Wolfson College Annexe, Linton Road Oxford OX2 6UD 3 Oxford Vaccine Group, Department of Paediatrics, University of Oxford, Oxford OX3 7LJ.
Abstract
BACKGROUND.: During seasonal influenza epidemics, 5-15% of the population are affected with an illness having a non-trivial mortality, morbidity and economic burden. Inactivated influenza vaccines are routinely used to prevent influenza infection, primarily by inducing humoral immunity. In addition, trivalent inactivated influenza vaccines have previously been shown to boost influenza-specific T-cell responses in a small percentage of adults. We investigate here the influenza-specific T-cell response, in children, one year after pandemic H1N1 vaccination and the ability to boost the T-cell response with trivalent inactivated influenza immunization. METHODS.: TPeripheral blood mononuclear cells (PBMCs) were isolated from children previously vaccinated with pandemic H1N1 vaccine, pre & post seasonal 2010-2011 TIV vaccination. Samples were analyzed by IFN-γ ELISpot for reactogencity toward internal influenza antigens (Nucleprotein (NP), Matrix protein 1 (M1), Nonstructural Protein 1 (NS1)). RESULTS.: Basal ex vivo T-cell responses to NP, M1 & NS1 measured by IFN-γ ELISpot assay were significantly higher in those children who had previously received an AS03B-adjuvanted split virion pandemic vaccine 12 months earlier rather than a non-adjuvanted whole virion vaccine. Boosting of these responses, 21 days after 2010/2011 seasonal TIV vaccination was observed regardless of age or prior pandemic vaccination regime, although boosting was greater in those groups with the lowest initial response. CONCLUSIONS.: We show here that children previously vaccinated with the 2009 pandemic H1N1 vaccine have measurable T-cell responses 1 year post vaccination. The magnitudes of these responses are dependent on both age of vaccinee and type of pandemic H1N1 vaccine used. Following 2010/2011 seasonal TIV vaccination these T-cell responses undergo a small but significant boost.
PMID:
22466328
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22466328