tetano
Editor, Senior Moderator
J Virol. 2011 Oct 26. [Epub ahead of print]
The TLR4-TRIF pathway protects against H5N1 influenza virus infection.
Shinya K, Ito M, Makino A, Tanaka M, Miyake K, Eisfeld AJ, Kawaoka Y.
Source
Department of Microbiology and Infectious Diseases, Graduate School of Medicine, Kobe University, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan.
Abstract
Pre-stimulation of the TLR4 pathway with lipopolysaccharide (LPS) protects mice from lethal infection with H5N1 influenza virus. Here, we reveal that the TLR4-TRIF pathway is required for this protective effect by using mice whose TLR4-related molecules were knocked-out. Microarray analysis of primary lung culture cells of LPS pre-treated mice infected with an H5N1 virus indicated that TLR3 mRNA was upregulated. Primary lung culture cells of TLR3 knockout mice showed no response to LPS pre-treatment against H5N1 virus infection, suggesting that TLR3 is also involved in the preventive effect of LPS. Our data suggest that the TLR4-TRIF axis has an important role in stimulating protective innate immunity against H5N1 influenza A virus infection and that TLR3 signaling is involved in this pathway.
PMID:
22031950
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22031950
The TLR4-TRIF pathway protects against H5N1 influenza virus infection.
Shinya K, Ito M, Makino A, Tanaka M, Miyake K, Eisfeld AJ, Kawaoka Y.
Source
Department of Microbiology and Infectious Diseases, Graduate School of Medicine, Kobe University, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan.
Abstract
Pre-stimulation of the TLR4 pathway with lipopolysaccharide (LPS) protects mice from lethal infection with H5N1 influenza virus. Here, we reveal that the TLR4-TRIF pathway is required for this protective effect by using mice whose TLR4-related molecules were knocked-out. Microarray analysis of primary lung culture cells of LPS pre-treated mice infected with an H5N1 virus indicated that TLR3 mRNA was upregulated. Primary lung culture cells of TLR3 knockout mice showed no response to LPS pre-treatment against H5N1 virus infection, suggesting that TLR3 is also involved in the preventive effect of LPS. Our data suggest that the TLR4-TRIF axis has an important role in stimulating protective innate immunity against H5N1 influenza A virus infection and that TLR3 signaling is involved in this pathway.
PMID:
22031950
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22031950