tetano
Editor, Senior Moderator
Antiviral Res. 2014 May 13. pii: S0166-3542(14)00131-4. doi: 10.1016/j.antiviral.2014.05.002. [Epub ahead of print]
The use of plethysmography in determining the severity of lung pathology in a mouse model of minimally lethal influenza virus infection.
Julander JG1, Kesler K2, Van Wettere AJ3, Morrey JD2, Smee DF2.
Author information
Abstract
To characterize the impact on lung function, we assessed plethysmography parameters in a course of infection with mouse-adapted A/Pennsylvania/14/2010 (H3N2) influenza virus. Several parameters, represented by enhanced pause (penh) and ratio of inspiratory/expiratory time (Ti/Te), were observed that had early (1-7 dpi) and robust changes regardless of virus challenge dose. Other parameters, characterized by tidal volume (TV), breathing frequency (freq) and end inspiratory pause (EIP), changed later (7-15 dpi) during the course of infection and had a virus challenge dose effect. A third category of lung function parameters, such as peak inspiratory flow, had early, virus challenge-independent changes followed by later changes that were challenge dependent. These parameters changed in a similar manner after infection with a non-mouse adapted virus, although the time-course of many parameters was delayed somewhat when compared with mouse-adapted virus. Histopathological assessment of lung samples corresponded with changes in lung function parameters. This study demonstrates the utility of plethysmography in assessing disease in a mouse model of mild influenza virus infection.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
H3N2 variant, Influenza virus, Lung function, Mice, Plethysmography
PMID:
24837607
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24837607
The use of plethysmography in determining the severity of lung pathology in a mouse model of minimally lethal influenza virus infection.
Julander JG1, Kesler K2, Van Wettere AJ3, Morrey JD2, Smee DF2.
Author information
Abstract
To characterize the impact on lung function, we assessed plethysmography parameters in a course of infection with mouse-adapted A/Pennsylvania/14/2010 (H3N2) influenza virus. Several parameters, represented by enhanced pause (penh) and ratio of inspiratory/expiratory time (Ti/Te), were observed that had early (1-7 dpi) and robust changes regardless of virus challenge dose. Other parameters, characterized by tidal volume (TV), breathing frequency (freq) and end inspiratory pause (EIP), changed later (7-15 dpi) during the course of infection and had a virus challenge dose effect. A third category of lung function parameters, such as peak inspiratory flow, had early, virus challenge-independent changes followed by later changes that were challenge dependent. These parameters changed in a similar manner after infection with a non-mouse adapted virus, although the time-course of many parameters was delayed somewhat when compared with mouse-adapted virus. Histopathological assessment of lung samples corresponded with changes in lung function parameters. This study demonstrates the utility of plethysmography in assessing disease in a mouse model of mild influenza virus infection.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
H3N2 variant, Influenza virus, Lung function, Mice, Plethysmography
PMID:
24837607
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24837607