tetano
Editor, Senior Moderator
Curr Opin Struct Biol. 2016 Jan 27;36:75-84. doi: 10.1016/j.sbi.2016.01.002. [Epub ahead of print]
[h=1]Towards a structural understanding of RNA synthesis by negative strand RNA viral polymerases.[/h] Reguera J[SUP]1[/SUP], Gerlach P[SUP]1[/SUP], Cusack S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Negative strand RNA viruses (NSVs), which may have segmented (sNSV) or non-segmented genomes (nsNSV) are responsible for numerous serious human infections such as Influenza, Measles, Rabies, Ebola, Crimean Congo Haemorrhagic Fever and Lassa Fever. Their RNA-dependent RNA polymerases transcribe and replicate the nucleoprotein coated viral genome within the context of a ribonucleoprotein particle. We review the first high resolution crystal and cryo-EM structures of representative NSV polymerases. The heterotrimeric Influenza and single-chain La Crosse orthobunyavirus polymerase structures (sNSV) show how specific recognition of both genome ends is achieved and is required for polymerase activation and how the sNSV specific 'cap-snatching' mechanism of transcription priming works. Vesicular Stomatitis Virus (nsNSV) polymerase shows a similar core architecture but has different flexibly linked C-terminal domains which perform mRNA cap synthesis. These structures pave the way for a more complete understanding of these complex, multifunctional machines which are also targets for anti-viral drug design.
Copyright ? 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.
PMID: 26826467 [PubMed - as supplied by publisher] Free full text
[h=1]Towards a structural understanding of RNA synthesis by negative strand RNA viral polymerases.[/h] Reguera J[SUP]1[/SUP], Gerlach P[SUP]1[/SUP], Cusack S[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Negative strand RNA viruses (NSVs), which may have segmented (sNSV) or non-segmented genomes (nsNSV) are responsible for numerous serious human infections such as Influenza, Measles, Rabies, Ebola, Crimean Congo Haemorrhagic Fever and Lassa Fever. Their RNA-dependent RNA polymerases transcribe and replicate the nucleoprotein coated viral genome within the context of a ribonucleoprotein particle. We review the first high resolution crystal and cryo-EM structures of representative NSV polymerases. The heterotrimeric Influenza and single-chain La Crosse orthobunyavirus polymerase structures (sNSV) show how specific recognition of both genome ends is achieved and is required for polymerase activation and how the sNSV specific 'cap-snatching' mechanism of transcription priming works. Vesicular Stomatitis Virus (nsNSV) polymerase shows a similar core architecture but has different flexibly linked C-terminal domains which perform mRNA cap synthesis. These structures pave the way for a more complete understanding of these complex, multifunctional machines which are also targets for anti-viral drug design.
Copyright ? 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.
PMID: 26826467 [PubMed - as supplied by publisher] Free full text