tetano
Editor, Senior Moderator
J Virol. 2013 Jul 17. [Epub ahead of print]
Uncoupling of influenza A virus transcription and replication through mutation of the unpaired adenosine in the vRNA promoter.
Te Velthuis AJ, Turrell L, Vreede FT, Fodor E.
Source
University of Oxford, Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, United Kingdom.
Abstract
Transcription and replication of the influenza A virus RNA genome are mediated by the viral RNA polymerase from a promoter consisting of the partially base-paired 3' and 5' termini of viral genome segments. Here we show that transcription and replication can be uncoupled by mutation of an unpaired adenosine in the 5'-strand of the promoter. This residue is important for transcription but not replication by being essential for the cap-binding activity of the RNA polymerase.
PMID:
23864616
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23864616
Uncoupling of influenza A virus transcription and replication through mutation of the unpaired adenosine in the vRNA promoter.
Te Velthuis AJ, Turrell L, Vreede FT, Fodor E.
Source
University of Oxford, Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, United Kingdom.
Abstract
Transcription and replication of the influenza A virus RNA genome are mediated by the viral RNA polymerase from a promoter consisting of the partially base-paired 3' and 5' termini of viral genome segments. Here we show that transcription and replication can be uncoupled by mutation of an unpaired adenosine in the 5'-strand of the promoter. This residue is important for transcription but not replication by being essential for the cap-binding activity of the RNA polymerase.
PMID:
23864616
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23864616